磷脂膜部分磁取向的31P核磁共振首次谱矩研究。
31P NMR first spectral moment study of the partial magnetic orientation of phospholipid membranes.
作者信息
Picard F, Paquet M J, Levesque J, Bélanger A, Auger M
机构信息
Département de Chimie, Centre de Recherche en Sciences et Ingénierie des Macromolécules, Université Laval, Québec, Québec G1K 7P4, Canada.
出版信息
Biophys J. 1999 Aug;77(2):888-902. doi: 10.1016/S0006-3495(99)76940-7.
Abstract
Structural data can be obtained on proteins inserted in magnetically oriented phospholipid membranes such as bicelles, which are most often made of a mixture of long and short chain phosphatidylcholine. Possible shapes for these magnetically oriented membranes have been postulated in the literature, such as discoidal structures with a thickness of one bilayer and with the short acyl chain phosphatidylcholine on the edges. In the present paper, a geometrical study of these oriented structures is done to determine the validity of this model. The method used is based on the determination of the first spectral moment of solid-state (31)P nuclear magnetic resonance spectra. From this first moment, an order parameter is defined that allows a quantitative analysis of partially oriented spectra. The validity of this method is demonstrated in the present study for oriented samples made of DMPC, DMPC:DHPC, DMPC:DHPC:gramicidin A and adriamycin:cardiolipin.
摘要
可以获取插入磁性取向磷脂膜（如双分子层）中的蛋白质的结构数据，双分子层通常由长链和短链磷脂酰胆碱的混合物制成。文献中已推测出这些磁性取向膜的可能形状，例如厚度为一个双层且边缘带有短酰基链磷脂酰胆碱的盘状结构。在本文中，对这些取向结构进行了几何研究，以确定该模型的有效性。所使用的方法基于固态（31）P核磁共振谱的第一谱矩的测定。从这个第一矩定义了一个序参量，它允许对部分取向谱进行定量分析。在本研究中，该方法对由二肉豆蔻酰磷脂酰胆碱（DMPC）、DMPC:二己酰磷脂酰胆碱（DHPC）、DMPC:DHPC:短杆菌肽A以及阿霉素:心磷脂制成的取向样品的有效性得到了证明。
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