从磁性部分取向脂质双层的2H NMR谱确定取向序参数。
Determination of orientational order parameters from 2H NMR spectra of magnetically partially oriented lipid bilayers.
作者信息
Schäfer H, Mädler B, Sternin E
机构信息
Department of Physics, Brock University, St. Catharines, Ontario, Canada.
出版信息
Biophys J. 1998 Feb;74(2 Pt 1):1007-14. doi: 10.1016/S0006-3495(98)74025-1.
Abstract
The partial orientation of multilamellar vesicles (MLVs) in high magnetic fields is known to affect the shape of 2H NMR spectra. There are numerical methods for extracting either the orientational order parameters of lipid molecules for a random distribution of domain orientations in the sample, or the distribution of orientations for a known set of spectral anisotropies. A first attempt at determining the orientational order parameters in the presence of an unknown nonrandom distribution of orientations is presented. The numerical method is based on the Tikhonov regularization algorithm. It is tested using simulated partially oriented spectra. An experimental spectrum of a phospholipid-ether mixture in water is analyzed as an example. The experimental spectrum is consistent with an ellipsoidal shape of MLVs with a ratio of semiaxes of approximately 3.4.
摘要
已知多层层状囊泡（MLV）在高磁场中的部分取向会影响2H NMR谱的形状。存在一些数值方法，可用于提取样品中畴取向随机分布时脂质分子的取向序参数，或已知一组光谱各向异性时的取向分布。本文首次尝试在存在未知非随机取向分布的情况下确定取向序参数。该数值方法基于蒂洪诺夫正则化算法。使用模拟的部分取向光谱进行了测试。以水中磷脂 - 醚混合物的实验光谱为例进行了分析。实验光谱与半轴比约为3.4的MLV的椭球形一致。
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