Takashima H, Sakai H, Yanai T, Masegi T
Department of Veterinary Pathology, Faculty of Agriculture, Gifu University, Japan.
J Vet Med Sci. 1999 Jun;61(6):713-6. doi: 10.1292/jvms.61.713.
Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.
将附着在纤维蛋白上的胸膜肺炎放线杆菌（A. pleuropneumoniae）1、2、5或7型的全细胞固定于10%中性缓冲福尔马林中，然后石蜡包埋。将载玻片上的切片用抗生物素蛋白-生物素复合物免疫过氧化物酶（ABC）法染色。将试验血清作为一抗应用于切片。采用ABC法和补体结合（CF）试验检测针对胸膜肺炎放线杆菌（1、2、5和7型）的血清抗体。ABC试验和CF试验之间存在良好的相关性。目前的结果表明，免疫组织化学染色在检测和定量猪血清中的抗体方面与CF试验同样有用。
