Bossé J T, Friendship R, Rosendal S, Fenwick B W
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
J Vet Diagn Invest. 1993 Jul;5(3):359-62. doi: 10.1177/104063879300500309.
A mixed-antigen enzyme-linked immunosorbent assay (ELISA) containing serotype-specific polysaccharide antigens from serotypes 1, 5, and 7 of Actinobacillus pleuropneumoniae was developed. This ELISA was evaluated using sera from experimentally infected pigs. With a negative cutoff value of 0.250 (optical density at 405 nm), sensitivity and specificity were determined to be 96% and 99.5%, respectively. The ELISA was further evaluated using sera from commercial swine. Sows and suckling piglets were found to be the best age groups for detection of positive reactors to A. pleuropneumoniae. The mixed-antigen ELISA could be used in a herd health monitoring program for detection of A. pleuropneumoniae infections.
开发了一种混合抗原酶联免疫吸附测定法（ELISA），该方法包含胸膜肺炎放线杆菌血清型1、5和7的血清型特异性多糖抗原。使用来自实验感染猪的血清对该ELISA进行了评估。阴性临界值设定为0.250（405nm处的光密度），灵敏度和特异性分别确定为96%和99.5%。使用来自商业猪群的血清对该ELISA进行了进一步评估。发现母猪和哺乳仔猪是检测胸膜肺炎放线杆菌阳性反应动物的最佳年龄组。混合抗原ELISA可用于猪群健康监测计划，以检测胸膜肺炎放线杆菌感染。
