Lyubarev A E, Kurganov B I
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071, Russia.
Biochemistry (Mosc). 1999 Jul;64(7):832-8.
The model for thermal denaturation of proteins involving consecutive reversible and irreversible steps (Lumry and Eyring model) has been analyzed. The most general case, when equilibrium in the first step is established slowly in comparison with the rate of the second step and the heat effect value for the second step is either greater than or less than zero, has been considered. The theoretical dependences of excess heat capacity on temperature have been constructed. The variation of the shape of the theoretical curves with varied values of the enthalpy change for the second step, Arrhenius equation parameters for both steps, and the scanning rate has been studied.
对涉及连续可逆和不可逆步骤的蛋白质热变性模型（Lumry和Eyring模型）进行了分析。考虑了最一般的情况，即第一步中的平衡与第二步的速率相比建立得较慢，且第二步的热效应值大于或小于零。构建了过量热容随温度的理论依赖关系。研究了第二步焓变的不同值、两步的阿累尼乌斯方程参数以及扫描速率对理论曲线形状的影响。
