在英国从一头死产小牛中进行犬新孢子虫的体外分离。

In vitro isolation of Neospora caninum from a stillborn calf in the UK.

作者信息

Davison H C, Guy F, Trees A J, Ryce C, Ellis J T, Otter A, Jeffrey M, Simpson V R, Holt J J

机构信息

Veterinary Parasitology, Liverpool School of Tropical Medicine/Faculty of Veterinary Science, Pembroke Place, Liverpool, L3 5QA, UK.

出版信息

Res Vet Sci. 1999 Aug;67(1):103-5. doi: 10.1053/rvsc.1998.0272.

DOI:10.1053/rvsc.1998.0272

PMID:10425249

Abstract

Neospora caninum was isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LivB1) is the first to be obtained from cattle in the United Kingdom and was confirmed as N. caninum by immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS1) sequence analysis. Differences were found between NC-LivB1, other bovine isolates and canine isolates of N. caninum and closely related protozoal parasites, using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR) techniques.

摘要

从一头死产小牛的大脑中，在Vero细胞培养物中分离出了犬新孢子虫。该分离株（命名为NC-LivB1）是英国首次从牛身上获得的，通过用特异性抗体进行免疫荧光检测以及内部转录间隔区1（ITS1）序列分析，被确认为犬新孢子虫。利用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）技术，发现NC-LivB1、犬新孢子虫的其他牛分离株和犬分离株以及密切相关的原生动物寄生虫之间存在差异。