一株瑞典犬新孢子虫牛分离株的特性鉴定

Characterization of a Swedish bovine isolate of Neospora caninum.

作者信息

Stenlund S, Björkman C, Holmdahl O J, Kindahl H, Uggla A

机构信息

Department of Parasitology, National Veterinary Institute, Uppsala, Sweden.

出版信息

Parasitol Res. 1997;83(3):214-9. doi: 10.1007/s004360050236.

DOI:10.1007/s004360050236

PMID:9089715

Abstract

The brain of a stillborn calf, seropositive to Neospora caninum and born to a seropositive cow, was homogenized and cultured on Vero cells, where growth of Neospora-like tachyzoites was detected after 8 weeks. The ultrastructural features of the new isolate (Nc-SweB1) corresponded to those of previously published Neospora isolates. In indirect immunofluorescence tests, antigens on Nc-SweB1 tachyzoites were recognized by antibodies raised to a canine N. caninum isolate (Nc-1) but not by antibodies to Toxoplasma gondii, Sarcocystis cruzi, S. tenella, Eimeria alabamensis, Babesia divergens, or B. motasi. Immunoblot analyses revealed no major antigenic difference between Nc-SweB1 and Nc-1, whereas several differences were seen between Nc-SweB1 and protozoa related to N. caninum. The sequences of 16S-like rRNA and the internal transcribed spacer 1 of Nc-SweB1 revealed complete homology with corresponding sequences of two canine N. caninum isolates. Thus, no dissimilarity between Nc-SweB1 and the canine isolates was found, confirming that Nc-SweB1 is N. caninum and suggesting that Neospora-like organisms isolated from cattle are indeed N. caninum.

摘要

一头死产小牛的大脑，其对犬新孢子虫血清呈阳性，且出生于血清呈阳性的母牛，将该大脑进行匀浆并在Vero细胞上培养，8周后检测到类似新孢子虫速殖子的生长。新分离株（Nc-SweB1）的超微结构特征与先前发表的新孢子虫分离株一致。在间接免疫荧光试验中，Nc-SweB1速殖子上的抗原可被针对犬新孢子虫分离株（Nc-1）产生的抗体识别，但不能被针对刚地弓形虫、克鲁斯肉孢子虫、柔嫩艾美耳球虫、阿拉巴马艾美耳球虫、分歧巴贝斯虫或莫氏巴贝斯虫的抗体识别。免疫印迹分析显示Nc-SweB1与Nc-1之间无主要抗原差异，而Nc-SweB1与新孢子虫相关原生动物之间存在一些差异。Nc-SweB1的16S样rRNA和内部转录间隔区1序列与两种犬新孢子虫分离株的相应序列完全同源。因此，未发现Nc-SweB1与犬分离株之间存在差异，证实Nc-SweB1为犬新孢子虫，并表明从牛分离出的类似新孢子虫的生物体确实是犬新孢子虫。