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MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐] 测定法是一种用于比色法测定真菌细胞密度的快速且可靠的方法。

The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities.

作者信息

Freimoser F M, Jakob C A, Aebi M, Tuor U

机构信息

Institut fr Mikrobiologie, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.

出版信息

Appl Environ Microbiol. 1999 Aug;65(8):3727-9. doi: 10.1128/AEM.65.8.3727-3729.1999.

DOI:10.1128/AEM.65.8.3727-3729.1999
PMID:10427074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC91559/
Abstract

The entomopathogenic fungus Neozygites parvispora (Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies in a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for the cultivation of this fungus, we were looking for a rapid and quantitative method to estimate the number of living cells in small volumes of liquid culture. A colorimetric method for the determination of cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] proved to be more accurate and timesaving than conventional hemocytometer counting.

摘要

昆虫病原真菌小孢新接霉（虫霉目：接合菌纲）在复合培养基中体外生长时呈不规则杆状菌丝体。为了简化培养基成分并确定用于该真菌培养的促生长化合物，我们在寻找一种快速且定量的方法来估算少量液体培养物中的活细胞数量。使用MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐] 测定细胞密度的比色法被证明比传统的血细胞计数器计数更准确且更省时。

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