Cantalejo M J, Torondel P, Amate L, Carrasco J M, Hernández E
Department of Biotechnology, Polytechnic University of Valencia, Spain.
J Basic Microbiol. 1999;39(3):143-53. doi: 10.1002/(sici)1521-4028(199906)39:3<143::aid-jobm143>3.0.co;2-u.
A group of 49 strains of Fusarium sp. isolated from different Spanish samples of cereals and mixed feedstuffs were screened for their ability to produce trichothecenes like T-2 toxin (T-2), HT-2 toxin (HT-2), diacetoxyscirpenol (DAS) and deoxynivalenol (DN), as well as other mycotoxin produced by Fusarium named fusarin C. The production of these mycotoxins was analyzed by means of spectrophotometry, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography (GC). Results showed that from 19 Fusarium strains in which cultures trichothecene production was detected, 15 were HT-2 producers, 9 T-2 producers, 14 DAS producers and 10 DN producers. On the other hand, from 28 Fusarium strains in which cultures fusarin C production was detected, 22 were low fusarin C producers (ranged from 0.04 to 1 microgram/l ICI medium), 5 Fusarium strains were intermediate-level producers (ranged from 1 to 10 micrograms/l ICI medium) and one Fusarium strain produced 240 micrograms/l ICI medium. The identified Fusarium strains that produced trichothecenes and fusarin C were F. moniliforme and F. oxysporum.
从西班牙不同谷物和混合饲料样本中分离出49株镰刀菌属菌株，对其产生诸如T-2毒素（T-2）、HT-2毒素（HT-2）、二乙酰基镳草镰刀菌烯醇（DAS）和脱氧雪腐镰刀菌烯醇（DN）等单端孢霉烯族毒素以及镰刀菌属产生的另一种霉菌毒素镰刀菌素C的能力进行了筛选。通过分光光度法、薄层色谱法（TLC）、高效液相色谱法（HPLC）和气相色谱法（GC）分析了这些霉菌毒素的产生情况。结果显示，在检测到产单端孢霉烯族毒素的19株镰刀菌菌株中，15株产生HT-2毒素，9株产生T-2毒素，14株产生DAS毒素，10株产生DN毒素。另一方面，在检测到产镰刀菌素C的28株镰刀菌菌株中，22株为低产镰刀菌素C菌株（在ICI培养基中的产量范围为0.04至1微克/升），5株镰刀菌菌株为中产菌株（在ICI培养基中的产量范围为1至10微克/升），1株镰刀菌菌株在ICI培养基中的产量为240微克/升。鉴定出的产单端孢霉烯族毒素和镰刀菌素C的镰刀菌菌株为串珠镰刀菌和尖孢镰刀菌。
