布氏锥虫核糖体蛋白L24编码cDNA的克隆与鉴定

Cloning and characterisation of cDNA encoding the Trypanosoma brucei ribosomal protein L24.

作者信息

Read L K, Militello K T, Nerantzakis G E

机构信息

Department of Microbiology and Center for Microbial Pathogenesis, SUNY Buffalo School of Medicine, NY 14214, USA.

出版信息

Int J Parasitol. 1999 Apr;29(4):601-5. doi: 10.1016/s0020-7519(99)00011-9.

DOI:10.1016/s0020-7519(99)00011-9

PMID:10428636

Abstract

A cDNA encoding ribosomal protein L24 was amplified by PCR from the protozoan parasite, Trypanosoma brucei. The 621 nucleotide cDNA had an open reading frame of 375 nucleotides, predicting a highly basic protein of 125 aa. Database searches revealed 33-40% identity between the T. brucei RPL24 protein and several eukaryotic RPL24 homologues. Southern blot analysis indicated that the gene was present as a single copy, and a transcript of approximately 620 nucleotides was detected in procyclic forms of the parasite. Interestingly, T. brucei PRL24 is the smallest eukaryotic RPL24 protein described to date. It is also the most divergent of the known kinetoplastid ribosomal proteins.

摘要

通过聚合酶链反应（PCR）从原生动物寄生虫布氏锥虫中扩增出编码核糖体蛋白L24的互补DNA（cDNA）。这个621个核苷酸的cDNA有一个375个核苷酸的开放阅读框，预测编码一个由125个氨基酸组成的高度碱性蛋白。数据库搜索显示，布氏锥虫RPL24蛋白与几种真核生物RPL24同源物之间的同一性为33%-40%。Southern印迹分析表明该基因以单拷贝形式存在，并且在该寄生虫的前循环形式中检测到一个约620个核苷酸的转录本。有趣的是，布氏锥虫PRL24是迄今为止描述的最小的真核生物RPL24蛋白。它也是已知的动基体核糖体蛋白中差异最大的。