Hernández R, Palacios S, Herrera J, Martínez-Calvillo S, López I
Department of Molecular Biology, Universidad Nacional Autónoma de México (UNAM), México, DF, Mexico.
Biochim Biophys Acta. 1998 Feb 11;1395(3):321-5. doi: 10.1016/s0167-4781(97)00224-8.
Two cDNA clones encoding a protein homologous to ribosomal protein S4 from the protozoan parasite Trypanosoma cruzi were isolated and characterized. Both clones potentially encode for an identical basic protein of 273 amino acids. Sequence comparisons with other species indicate that this protein is highly conserved. Hybridization studies are consistent with the occurrence of two genes in T. cruzi encoding this ribosomal protein. An RNA of approximately 1 kb was present in both exponentially growing and stationary phase derived epimastigotes, and no significant differences were detected in its steady state concentration.
分离并鉴定了两个编码与原生动物寄生虫克氏锥虫核糖体蛋白S4同源的蛋白质的cDNA克隆。两个克隆都可能编码一种相同的含273个氨基酸的碱性蛋白。与其他物种的序列比较表明，该蛋白高度保守。杂交研究表明，克氏锥虫中有两个基因编码这种核糖体蛋白。在指数生长期和稳定期的副鞭毛体中均存在约1 kb的RNA，其稳态浓度未检测到显著差异。
