Ueki N, Seki N, Yano K, Masuho Y, Saito T, Muramatsu M
Biological Technology Laboratory, Helix Research Institute, Chiba, Japan.
J Hum Genet. 1999;44(4):256-60. doi: 10.1007/s100380050155.
Rat ENH (Enigma homolog) is a LIM domain protein that associates with protein kinase C in an isoform-specific manner. We have identified a human cDNA which shares a significant sequence homology with rat ENH. The isolated cDNA clone, designated human ENH (hENH), was 3287 bp in length and encoded a predicted protein of 596 amino acids which had 88% overall identity to rat ENH protein. Northern blot analysis revealed that 1.9 kb of the hENH messenger RNA was predominantly expressed in heart and skeletal muscle, while 5.6 kb of the hENH messenger RNA was ubiquitously expressed in various human tissues. The chromosomal location of the gene was determined on chromosome 4q22 region, between markers WI-2900 and WI-3273, by polymerase chain reaction (PCR)-based analyses using both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid mapping panel.
大鼠ENH（Enigma同源物）是一种LIM结构域蛋白，它以同工型特异性方式与蛋白激酶C结合。我们鉴定出了一种与大鼠ENH具有显著序列同源性的人类cDNA。分离得到的cDNA克隆，命名为人类ENH（hENH），长度为3287 bp，编码一个预测的由596个氨基酸组成的蛋白质，该蛋白质与大鼠ENH蛋白的总体一致性为88%。Northern印迹分析显示，1.9 kb的hENH信使RNA主要在心脏和骨骼肌中表达，而5.6 kb的hENH信使RNA在各种人类组织中普遍表达。通过使用人类/啮齿动物单染色体杂交细胞系和辐射杂种图谱绘制板进行基于聚合酶链反应（PCR）的分析，确定了该基因在4号染色体q22区域的染色体定位，位于标记WI-2900和WI-3273之间。
