Gabrielska J, Oszmiański J, Komorowska M, Langner M
Department of Physics and Biophysics, Agricultural University, Wrocław, Poland.
Z Naturforsch C J Biosci. 1999 May-Jun;54(5-6):319-24. doi: 10.1515/znc-1999-5-605.
The antioxidative activity of three anthocyanin pigments, extracted from the fruits of chokeberry, honeysuckle and sloe, were studied. Lipid oxidation in the liposome membrane, induced by UV radiation, was evaluated with a thiobarbituric acid-reactive substances assay. The antioxidant efficiency of the studied compounds follows this sequence: chokeberry > sloe > honeysuckle. The extract concentrations at which a 50% reduction of phosphatidylcholine oxidation was observed, were respectively: 48, 54 and 60 mg/l. The end products of lipid membrane oxidation were evaluated using HPLC. It was found that the antioxidative potency of anthocyanin extracts is concentration-dependent. As shown by EPR technique the efficiency of the extracts to eliminate free radicals from the solution follows the order of the antioxidant activity.
对从黑果腺肋花楸、金银花和黑刺李果实中提取的三种花青素色素的抗氧化活性进行了研究。通过硫代巴比妥酸反应性物质测定法评估紫外线辐射诱导的脂质体膜中的脂质氧化。所研究化合物的抗氧化效率遵循以下顺序:黑果腺肋花楸>黑刺李>金银花。观察到磷脂酰胆碱氧化减少50%时的提取物浓度分别为:48、54和60 mg/l。使用高效液相色谱法评估脂质膜氧化的终产物。发现花青素提取物的抗氧化能力与浓度有关。电子顺磁共振技术表明,提取物从溶液中清除自由基的效率遵循抗氧化活性的顺序。