Brantner A H, Males Z
Institute of Pharmacognosy, University of Graz, Austria.
J Ethnopharmacol. 1999 Aug;66(2):175-9. doi: 10.1016/s0378-8741(98)00180-9.
Because the flavonoid glycosides can be considered as suitable compounds for the quality assessment of Paliurus spina-christi Mill., a high-performance liquid-chromatographic method for the separation and quantification of these compounds in methanolic extracts of the different plant parts (leaves, flowers, fruits) is described. The system used is a reversed-phase column and gradient elution with water-phosphoric acid-acetonitrile. The analysis showed the flavonoid contents of the different plant parts and the different composition of the flavonoid pattern. The highest flavonoid content was found in the leaves in June and July. No significant influence of growing site or year of harvesting on the flavonoid content was observed. As quercetin 3-O-rhamnoglucoside 7-O-rhamnoside and rutin are the main flavonoid compounds present in all plant parts investigated, it is suggested that these compounds should be used for the quality assessment of Paliurus spina-christi.
由于黄酮苷可被视为用于马甲子质量评估的合适化合物，本文描述了一种高效液相色谱法，用于分离和定量不同植物部位（叶、花、果实）甲醇提取物中的这些化合物。所使用的系统是反相柱，并采用水 - 磷酸 - 乙腈进行梯度洗脱。分析显示了不同植物部位的黄酮含量以及黄酮图谱的不同组成。6月和7月时叶片中的黄酮含量最高。未观察到生长地点或收获年份对黄酮含量有显著影响。由于槲皮素3 - O - 鼠李糖基葡萄糖苷7 - O - 鼠李糖苷和芦丁是所有研究植物部位中主要的黄酮类化合物，因此建议将这些化合物用于马甲子的质量评估。
