Goo Hyun Ryul, Choi Jae Sue, Na Dong Hee
College of Pharmacy, Kyungsung University, Busan 608-736, Korea.
Arch Pharm Res. 2009 Feb;32(2):201-6. doi: 10.1007/s12272-009-1136-y. Epub 2009 Mar 13.
The purpose of this study was to develop reversed-phase HPLC method for the simultaneous determination of the flavonoids from the leaves of Nelumbo nucifera, which have been known to exhibit antioxidant, anti-HIV, antihyperlipidemic and antiobesity effects. HPLC separation was achieved on C(18) column using gradient elution with mobile phase consisting of acetonitrile-water containing 0.1% formic acid. The separated peaks were identified by electrospray ionization mass spectrometry. The HPLC method was validated and applied for the simultaneous determination of the bioactive flavonoids from the leaves of Nelumbo nucifera. In the methanol extract, six flavonoids, quercetin, rutin, quercetin 3-O-beta-D-galactopyranoside (Qc-3-Gal), quercetin 3-O-beta-D-glucopyranoside (Qc-3-Glc), quercetin 3-O-beta-D-glucuronide (Qc-3-Gln) and quercetin 3-O-alpha-arabinopyranosyl-(1-->2)-beta-galactopyranoside (Qc-3-AraGal), were identified. Among them, Qc-3-Glc and Qc-3-Gln were found to be major component in the methanol extract of Nelumbo nucifera leaves.
本研究的目的是开发一种反相高效液相色谱法，用于同时测定莲（Nelumbo nucifera）叶中的黄酮类化合物，已知这些黄酮类化合物具有抗氧化、抗艾滋病毒、抗高血脂和抗肥胖作用。使用由含0.1%甲酸的乙腈-水组成的流动相进行梯度洗脱，在C(18)柱上实现高效液相色谱分离。通过电喷雾电离质谱法鉴定分离出的峰。对该高效液相色谱法进行了验证，并将其应用于同时测定莲（Nelumbo nucifera）叶中的生物活性黄酮类化合物。在甲醇提取物中，鉴定出了六种黄酮类化合物，即槲皮素、芦丁、槲皮素3-O-β-D-吡喃半乳糖苷（Qc-3-Gal）、槲皮素3-O-β-D-吡喃葡萄糖苷（Qc-3-Glc）、槲皮素3-O-β-D-葡萄糖醛酸苷（Qc-3-Gln）和槲皮素3-O-α-L-阿拉伯吡喃糖基-(1→2)-β-D-吡喃半乳糖苷（Qc-3-AraGal）。其中，Qc-3-Glc和Qc-3-Gln被发现是莲（Nelumbo nucifera）叶甲醇提取物中的主要成分。
