Ishii Y, Miyanaga Y, Shimojo H, Ushida T, Tateishi T
Institute of Health and Sport Sciences, University of Tsukuba, Tsukuba, Japan.
Tissue Eng. 1999 Jun;5(3):279-86. doi: 10.1089/ten.1999.5.279.
In the present study, we examined the effect of intermittent exposure to hyperbaric oxygen (HBO) on experimentally induced tendon laceration in the right hind limb of 56 male Wistar rats. We compared animals treated by HBO (2 atmospheres absolute [ATA] 100% O(2) x 60 min x once per day) with control animals (without HBO) at 3, 7, 14, and 28 days after wound creation. Histology, and pro-alpha1 (I) mRNA by Northern hybridization, pro-alpha1 (III) mRNA, transforming growth factor-beta1 (TGF-beta1) mRNA by RT-PCR were studied. Our results indicate the use of HBO influences the pro-a1 (I) mRNA expression. The level of pro-alpha1 (I) mRNA was especially increased at 7 14 days after laceration by HBO. These results suggest that intermittent oxygen exposure may play a significant role in collagen synthesis and be beneficial for producing the extracellular matrices in tissue engineering.
在本研究中，我们检测了间歇性暴露于高压氧（HBO）对56只雄性Wistar大鼠右后肢实验性诱导肌腱撕裂的影响。在伤口形成后的第3、7、14和28天，我们将接受HBO治疗的动物（2个绝对大气压[ATA] 100% O₂，60分钟，每天1次）与对照动物（未接受HBO）进行比较。研究了组织学，通过Northern杂交检测前α1（I）mRNA，通过RT-PCR检测前α1（III）mRNA、转化生长因子-β1（TGF-β1）mRNA。我们的结果表明，使用HBO会影响前α1（I）mRNA的表达。在撕裂伤后7至14天，前α1（I）mRNA的水平尤其升高。这些结果表明，间歇性氧暴露可能在胶原蛋白合成中起重要作用，并且有利于在组织工程中产生细胞外基质。
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