在干燥综合征中应用自体血清治疗干眼症。
Treatment of dry eye by autologous serum application in Sjögren's syndrome.
作者信息
Tsubota K, Goto E, Fujita H, Ono M, Inoue H, Saito I, Shimmura S
机构信息
Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
出版信息
Br J Ophthalmol. 1999 Apr;83(4):390-5. doi: 10.1136/bjo.83.4.390.
AIM
To evaluate the efficacy of autologous serum application for the treatment of dry eye in Sjögren's syndrome.
METHODS
The stability of essential components (EGF, vitamin A, and TGF-beta) in preserved serum were examined following preservation at 4 degrees C and -20 degrees C. In a primary clinical trial, 12 patients with Sjögren's syndrome were treated with autologous serum (diluted to 20% with sterile saline) for 4 weeks, and vital staining of the ocular surface was compared before and after treatment. The effects of serum on mucin (MUC-1) expression were observed in cultured conjunctival epithelial cells in vitro.
RESULTS
EGF, vitamin A, and TGF-beta were well preserved for up to 1 month in the refrigerator at 4 degrees C and up to 3 months in the freezer at -20 degrees C. Rose bengal and fluorescein scores improved significantly from the initial scores of 5.3 and 5.6 to 1.7 and 2.5 after 4 weeks, respectively. The additive effect of human serum for cultured conjunctival epithelial cells showed significant MUC-1 upregulation on the cell surface.
CONCLUSION
Autologous serum application is a safe and efficient way to provide essential components to the ocular surface in the treatment of dry eye associated with Sjögren's syndrome.
目的
评估应用自体血清治疗干燥综合征干眼的疗效。
方法
检测保存血清中主要成分（表皮生长因子、维生素A和转化生长因子-β）在4℃和-20℃保存后的稳定性。在一项初步临床试验中，12例干燥综合征患者用自体血清（用无菌生理盐水稀释至20%）治疗4周，比较治疗前后眼表活体染色情况。在体外培养的结膜上皮细胞中观察血清对黏蛋白（MUC-1）表达的影响。
结果
表皮生长因子、维生素A和转化生长因子-β在4℃冰箱中可保存长达1个月，在-20℃冷冻室中可保存长达3个月。4周后，孟加拉玫瑰红和荧光素评分分别从初始的5.3和5.6显著改善至1.7和2.5。人血清对培养的结膜上皮细胞的相加作用显示细胞表面MUC-1显著上调。
结论
应用自体血清是为干燥综合征相关干眼的眼表提供主要成分的一种安全有效的方法。
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