Yamada H, Miyauchi S, Hotta H, Morita M, Yoshihara Y, Kikuchi T, Fujikawa K
Department of Orthopaedic Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359, Japan.
J Orthop Sci. 1999;4(4):250-4. doi: 10.1007/s007760050100.
We determined the levels of chondroitin sulfate (CS) isomers in the synovial fluid of patients with hip osteoarthritis (OA) to investigate their significance as markers reflecting extracellular matrix metabolism in joint tissues. A cross-sectional study of synovial fluid aspirated from 50 hip joints of 50 patients with OA was performed. Concentrations of chondroitin-4-sulfate (C4S) and chondroitin-6-sulfate (C6S) were determined by high-performance liquid chromatography. Levels of each marker molecule were investigated in relation to age and radiological stage of the disease. In all disease stages, the dominant CS isomer in synovial fluid was C6S. There was a significant negative correlation between levels of C6S and age. A significant inverse correlation was also observed between the ratio of C6S to C4S and age. Results of analysis of covariance in which age was covariate showed that the ratio of C6S to C4S in advanced and terminal stage OA was lower than that in early stage OA. The present results indicate that the ratio of CS isomers in synovial fluid in hip OA varies with the severity of disease. These molecules in synovial fluid may serve as a useful marker reflecting extracellular matrix metabolism in OA.
我们测定了髋骨关节炎（OA）患者滑液中硫酸软骨素（CS）异构体的水平，以研究其作为反映关节组织细胞外基质代谢标志物的意义。对50例OA患者的50个髋关节抽取的滑液进行了横断面研究。采用高效液相色谱法测定硫酸软骨素-4-硫酸盐（C4S）和硫酸软骨素-6-硫酸盐（C6S）的浓度。研究了每种标志物分子水平与疾病年龄和放射学分期的关系。在所有疾病阶段，滑液中占主导地位的CS异构体是C6S。C6S水平与年龄之间存在显著负相关。C6S与C4S的比值与年龄之间也观察到显著的负相关。以年龄为协变量的协方差分析结果表明，晚期和终末期OA中C6S与C4S的比值低于早期OA。目前的结果表明，髋OA滑液中CS异构体的比值随疾病严重程度而变化。滑液中的这些分子可能作为反映OA细胞外基质代谢的有用标志物。
