Yang D, Yuan Z, Li C
Deptartment of Endocrinology, First Teaching Hospital, Beijing Medical University.
Zhonghua Nei Ke Za Zhi. 1997 Oct;36(10):685-8.
To investigate the expression of thyrotropin (TSH) receptor gene in autoimmune thyroid disease and the relationship with expression of thyroid peroxidase (TPO) and thyroglobulin (TG) genes, we examined TSH receptor, TPO and TG gene expression levels in 10 human thyroid tissues by Northern blot analysis, including 7 Graves disease (GD), 2 Hashimoto disease (HT), and 1 normal thyroid tissue. The expression levels of TSH-R gene and TG/TPO gene were different between GD group and HT group. In GD group, TSH-R gene expression was closely correlated to the expression of TPO gene (r = 0.882; P < 0.01) and TG gene (r = 0.723; P < 0.05). The gene expression levels in GD were higher than those in HT and control. In HT group, TPO, TG gene expression levels were relatively lower while TSH-R gene expression levels were still in normal range. The difference in gene expression is possibly related to the types of autoimmune antibodies and various dripping rates.
为研究促甲状腺激素（TSH）受体基因在自身免疫性甲状腺疾病中的表达及其与甲状腺过氧化物酶（TPO）和甲状腺球蛋白（TG）基因表达的关系，我们采用Northern印迹分析法检测了10例人甲状腺组织中TSH受体、TPO和TG基因的表达水平，其中包括7例Graves病（GD）、2例桥本氏病（HT）和1例正常甲状腺组织。GD组和HT组之间TSH-R基因以及TG/TPO基因的表达水平存在差异。在GD组中，TSH-R基因表达与TPO基因表达密切相关（r = 0.882；P < 0.01），与TG基因表达也密切相关（r = 0.723；P < 0.05）。GD组中的基因表达水平高于HT组和对照组。在HT组中，TPO、TG基因表达水平相对较低，而TSH-R基因表达水平仍在正常范围内。基因表达的差异可能与自身免疫抗体的类型和不同的滴度有关。
