人甲状腺肿瘤组织中促甲状腺激素受体、甲状腺球蛋白和甲状腺过氧化物酶的mRNA水平。

The mRNA levels of thyrotropin receptor, thyroglobulin and thyroid peroxidase in neoplastic human thyroid tissues.

作者信息

Ohta K, Endo T, Onaya T

机构信息

Third Department of Internal Medicine, University of Yamanashi Medical School, Tamaho, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Feb 14;174(3):1148-53. doi: 10.1016/0006-291x(91)91540-s.

DOI:10.1016/0006-291x(91)91540-s

PMID:1996981

Abstract

Expression levels of thyrotropin receptor (TSH-R), thyroglobulin (Tg) and thyroid peroxidase (TPO) mRNA in normal and neoplastic human thyroid tissues (6 adenomas and 7 carcinomas) were investigated by Northern-blot and slot-blot analyses. We found that TSH-R mRNA levels were significantly lower in carcinoma tissues than in normal tissues. The levels of Tg mRNA were also significantly lowered in adenoma and carcinoma tissues as compared to normal tissues. In contrast, no significant difference was observed in the expression levels of TPO mRNA between these tissues. Furthermore, TSH-R mRNA levels were well-correlated with Tg mRNA levels in neoplastic tissues. These results suggest that mRNAs of TSH-R and Tg are expressed in relation to their degree of differentiation.

摘要

通过Northern印迹法和狭缝印迹分析法，研究了促甲状腺激素受体（TSH-R）、甲状腺球蛋白（Tg）和甲状腺过氧化物酶（TPO）mRNA在正常及肿瘤性人甲状腺组织（6例腺瘤和7例癌）中的表达水平。我们发现，癌组织中TSH-R mRNA水平显著低于正常组织。与正常组织相比，腺瘤和癌组织中Tg mRNA水平也显著降低。相反，这些组织中TPO mRNA的表达水平未观察到显著差异。此外，肿瘤组织中TSH-R mRNA水平与Tg mRNA水平高度相关。这些结果表明，TSH-R和Tg的mRNA表达与其分化程度有关。