Liu X, Zhu X Z
Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Zhongguo Yao Li Xue Bao. 1998 Nov;19(6):527-30.
To study the effect of basic fibroblast growth factor (bFGF) on acute forebrain ischemia-reperfusion injury in rats.
Both vertebral arteries were occluded by electrocautery and severe but transient bilateral cerebral ischemia was produced by clamping both common carotid arteries for 20 min in rats. The contents of dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) in striatum were determined by HPLC with electrochemic detector (HPLC-EC) at 3, 6, 12, 24 h after the onset of reperfusion. The contents of sodium, potassium in forebrain were determined by atomic absorption spectro-photometric method at 6-h reperfusion. Morphological changes of the striatum were also examined.
At 6-h reperfusion, the DA content in striatum decreased from (99 +/- 16) micrograms.g-1 (protein) in sham-operation group to (70 +/- 20) micrograms.g-1 (protein); the water and the sodium contents in forebrain increased from 77.34% +/- 0.19% to 79.6% +/- 0.6% and from (9.3 +/- 0.6) to (10.5 +/- 0.6) mg.g-1 (dry weight), respectively. bFGF (i.v. 45 micrograms.kg-1.h-1 for 3 h) from the start of reperfusion prevented the decrease of DA in striatum and the increases of water and sodium contents in forebrain. Histological examination also indicated that bFGF ameliorated the injury of neurons.
bFGF prevented the brain from injury of ischemia and reperfusion.
研究碱性成纤维细胞生长因子（bFGF）对大鼠急性前脑缺血再灌注损伤的影响。
采用电烙法闭塞双侧椎动脉，再夹闭双侧颈总动脉20分钟，造成大鼠严重但短暂的双侧脑缺血。在再灌注开始后3、6、12、24小时，用高效液相色谱电化学检测器（HPLC-EC）测定纹状体中多巴胺（DA）和3,4-二羟基苯乙酸（DOPAC）的含量。在再灌注6小时时，用原子吸收分光光度法测定前脑中钠、钾的含量。同时检查纹状体的形态学变化。
再灌注6小时时，纹状体中DA含量从假手术组的（99±16）微克·克-1（蛋白）降至（70±20）微克·克-1（蛋白）；前脑中水分和钠含量分别从77.34%±0.19%增至79.6%±0.6%，从（9.3±0.6）毫克·克-1（干重）增至（10.5±0.6）毫克·克-1（干重）。从再灌注开始给予bFGF（静脉注射45微克·千克-1·小时-1，共3小时）可防止纹状体中DA的减少以及前脑中水分和钠含量的增加。组织学检查也表明bFGF减轻了神经元损伤。
bFGF可预防脑缺血再灌注损伤。
