Xu W, Deng Y F
Guangdong Medical College, Zhanjiang, China.
Zhongguo Yao Li Xue Bao. 1996 Mar;17(2):161-3.
To study the protective effect of anisodine (Ani) on acute forebrain ischemia-reperfusion injury in rats.
Both vertebral arteries were occluded by electrocautery. Severe, but transient bilateral cerebral ischemia was produced by clamping both common carotid arteries in rats. Atomic absorption spectrophotometric and spectrophotometric methods were used to determine the contents of calcium and extravasated Evans blue (EB), respectively, remained in forebrain at 60-min recirculation after 30-min ischemia.
At 60-min recirculation, the brain calcium contents were increased from 112 +/- 6 micrograms/g brain dry weight in control (sham operation) group to 165 +/- 7 micrograms/g brain dry weight with marked increase of EB extravasation. Ani (2.5 mg.kg-1, i.p.), and scopolamine (Sco, 0.25 mg.kg-1, i.p.) decreased the elevated calcium and extravasated EB contents.
Ani prevented the brain from ischemia insults through reducing intracellular calcium accumulation resulted from ischemia and reperfusion.
研究山莨菪碱（Ani）对大鼠急性前脑缺血再灌注损伤的保护作用。
通过电烙术闭塞双侧椎动脉。夹闭大鼠双侧颈总动脉造成严重但短暂的双侧脑缺血。分别采用原子吸收分光光度法和分光光度法测定缺血30分钟后再灌注60分钟时前脑中钙和伊文思蓝（EB）外渗的含量。
再灌注60分钟时，脑钙含量从对照组（假手术组）的112±6微克/克脑干重增加到165±7微克/克脑干重，同时EB外渗显著增加。山莨菪碱（2.5毫克·千克-1，腹腔注射）和东莨菪碱（Sco，0.25毫克·千克-1，腹腔注射）降低了升高的钙含量和EB外渗量。
山莨菪碱通过减少缺血和再灌注导致的细胞内钙积累来保护脑免受缺血损伤。
