Collins R A, Howard C J, Duggan S E, Werling D
Division of Immunology and Pathology, Institute for Animal Health, Newbury, Berkshire, UK.
Vet Immunol Immunopathol. 1999 May;68(2-4):193-207. doi: 10.1016/s0165-2427(99)00020-3.
The effects of IL-12 on the responses of cattle peripheral blood mononuclear cells (PBMC) to bovine respiratory syncytial virus (BRSV) antigen and ovalbumin (OVA) were tested, in vitro. IL-12 did not affect the proliferative responses of PBMC to these antigens but markedly accelerated and augmented the level of IFNgamma secreted. When tested on lymphoblasts rather than resting T-cells IL-12 also enhanced proliferation. In contrast IL-4 and, to greater extent, IL-10 inhibited the response. The effect of IL-12 on IFNgamma synthesis was confirmed at the level of IFNgamma. mRNA expression using Taqman PCR. CD4 and CD8 T-cell populations produced IFNgamma, however, CD4 T-cells comprised the largest contributors to the IFNgamma production. Gamma/delta T-cells did not contribute markedly. A comparison of the species cross-reactivity showed bovine IL-12 was also active in the human system. This study shows that antigen-driven responses in cattle can be significantly influenced by exogenous cytokines and suggests the IL-12/IL-10 balance is crucial for regulation of IFNgamma.
体外测试了白细胞介素-12（IL-12）对牛外周血单核细胞（PBMC）对牛呼吸道合胞病毒（BRSV）抗原和卵清蛋白（OVA）反应的影响。IL-12不影响PBMC对这些抗原的增殖反应，但显著加速并增强了γ干扰素（IFNγ）的分泌水平。当在淋巴母细胞而非静息T细胞上进行测试时，IL-12也增强了增殖。相比之下，IL-4以及在更大程度上IL-10抑制了该反应。使用Taqman PCR在IFNγ mRNA表达水平上证实了IL-12对IFNγ合成的影响。CD4和CD8 T细胞群体产生IFNγ，然而，CD4 T细胞是IFNγ产生的最大贡献者。γ/δ T细胞的贡献不显著。种间交叉反应性比较表明，牛IL-12在人类系统中也具有活性。这项研究表明，牛体内的抗原驱动反应可受到外源性细胞因子的显著影响，并提示IL-12/IL-10平衡对于IFNγ的调节至关重要。
