Larson M A, Kubisch H M
Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA.
Hum Reprod. 1999 Aug;14(8):2075-9. doi: 10.1093/humrep/14.8.2075.
The effects of culturing bovine embryos in groups were investigated. In the first experiment, 1000 oocytes were matured, fertilized and then cultured in groups of 40 in 25 microl of medium. From half of these groups, blastocysts were removed and cultured separately, while in the other half blastocysts were allowed to remain in the group culture microdrop. Blastocysts developed equally well in both groups, although hatching was reduced in those blastocysts removed from the culture droplet. In the second experiment, 1000 zygotes were cultured from the 8-cell stage to the blastocyst stage either individually or in groups of 40. Culture in groups increased the formation of blastocysts, the percentage of hatching blastocysts, the number of cells within blastocysts and the production of interferon-tau. In the final experiment, 1000 zygotes were cultured in groups up to the blastocyst stage. Two-thirds of these blastocysts were then cultured in groups of three, while the remaining blastocysts were cultured individually. Co-culture did not affect hatching or cell number but significantly elevated interferon-tau secretion. These results demonstrate that group culture either before or after blastocyst formation can alter the expression of a specific gene important for the establishment of pregnancy.
研究了牛胚胎群体培养的效果。在第一个实验中，1000个卵母细胞成熟、受精，然后以40个一组的方式在25微升培养基中培养。从这些组中的一半取出囊胚并单独培养，而另一半囊胚则留在群体培养微滴中。两组中的囊胚发育情况相同，尽管从培养微滴中取出的囊胚孵化率降低。在第二个实验中，1000个合子从8细胞阶段培养到囊胚阶段，要么单独培养，要么以40个一组的方式培养。群体培养增加了囊胚的形成、孵化囊胚的百分比、囊胚内的细胞数量以及干扰素τ的产生。在最后一个实验中，1000个合子群体培养至囊胚阶段。然后将其中三分之二的囊胚以三个一组的方式培养，其余的囊胚单独培养。共培养不影响孵化或细胞数量，但显著提高了干扰素τ的分泌。这些结果表明，在囊胚形成之前或之后进行群体培养可以改变对妊娠建立至关重要的特定基因的表达。
