Sugimura Satoshi, Akai Tomonori, Imai Kei
Department of Biological Production, Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Dai Nippon Printing Co., Ltd., Kashiwa, Chiba 277-0871, Japan.
J Reprod Dev. 2017 Aug 19;63(4):353-357. doi: 10.1262/jrd.2017-041. Epub 2017 May 26.
Conventionally, in vitro-fertilized (IVF) bovine embryos for transfer are morphologically evaluated at day 7-8 of embryo culture. This method is, however, subjective and results in unreliable selection. We previously described a novel selection system for IVF bovine blastocysts for transfer that traces the development of individual embryos with time-lapse monitoring in our specially developed microwell culture dishes (LinKID micro25). The system can noninvasively identify prognostic factors that reflect viability after transfer. By assessing a combination of identified prognostic factors -timing of the first cleavage; number of blastomeres at the end of the first cleavage; and number of blastomeres at the onset of lag-phase, which results in temporary developmental arrest during the fourth or fifth cell cycle- the pregnancy rate was improved over using conventional morphological evaluation. Time-lapse monitoring with LinKID micro25 could facilitate objective and reliable selection of healthy IVF bovine embryos. Here, we review the novel bovine embryo selection system that allows for prediction of viability after transfer.
传统上，用于移植的体外受精（IVF）牛胚胎在胚胎培养的第7至8天进行形态学评估。然而，这种方法具有主观性，会导致不可靠的选择。我们之前描述了一种用于移植的IVF牛囊胚的新型选择系统，该系统在我们专门开发的微孔培养皿（LinKID micro25）中通过延时监测追踪单个胚胎的发育情况。该系统可以无创地识别反映移植后活力的预后因素。通过评估所确定的预后因素的组合——第一次卵裂的时间；第一次卵裂结束时的卵裂球数量；以及滞后阶段开始时的卵裂球数量，滞后阶段会导致在第四或第五个细胞周期出现暂时的发育停滞——与使用传统形态学评估相比，妊娠率得到了提高。使用LinKID micro25进行延时监测可以促进对健康的IVF牛胚胎进行客观可靠的选择。在此，我们回顾了这种能够预测移植后活力的新型牛胚胎选择系统。
