Rahman M M, Guard-Petter J, Asokan K, Hughes C, Carlson R W
Complex Carbohydrate Research Center, The University of Georgia, Athens, Georgia 30602, USA.
J Biol Chem. 1999 Aug 13;274(33):22993-8. doi: 10.1074/jbc.274.33.22993.
Swarming by Proteus mirabilis is characterized by cycles of rapid and coordinated population migration across surfaces following differentiation of vegetative cells into elongated hyperflagellated swarm cells. It has been shown that surface colony expansion by the swarm cell population is facilitated by a colony migration factor (Cmf), a capsular polysaccharide (CPS) that also contributes to the uropathogenicity of P. mirabilis (Gygi, D., Rahman, M. M., Lai, H.-C., Carlson, R., Guard-Petter, J., and Hughes, C. (1995) Mol. Microbiol. 17, 1167-1175). In this report, the Cmf-CPS was extracted with hot water, precipitated with ethanol, and further purified by gel permeation chromatography. Its structure was established by glycosyl composition and linkage analyses, and by one- and two-dimensional NMR spectroscopy. The Cmf-CPS is composed of the following tetrasaccharide repeating unit. [see text]
奇异变形杆菌的群体游动特征为，营养细胞分化为细长的多鞭毛群体细胞后，细胞群体在表面进行快速且协调的迁移循环。研究表明，群体细胞群体对表面菌落的扩展受到一种菌落迁移因子（Cmf）的促进，Cmf是一种荚膜多糖（CPS），它也有助于奇异变形杆菌的尿路致病性（吉吉，D.，拉赫曼，M.M.，赖，H.-C.，卡尔森，R.，瓜德 - 佩特，J.，以及休斯，C.（1995年）《分子微生物学》17卷，第1167 - 1175页）。在本报告中，Cmf - CPS用热水提取，乙醇沉淀，并通过凝胶渗透色谱进一步纯化。其结构通过糖基组成和连接分析以及一维和二维核磁共振光谱确定。Cmf - CPS由以下四糖重复单元组成。[见正文]
