溶藻弧菌钠离子驱动鞭毛马达组件PomA和PomB之间的功能相互作用。
Functional interaction between PomA and PomB, the Na(+)-driven flagellar motor components of Vibrio alginolyticus.
作者信息
Yorimitsu T, Sato K, Asai Y, Kawagishi I, Homma M
机构信息
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan.
出版信息
J Bacteriol. 1999 Aug;181(16):5103-6. doi: 10.1128/JB.181.16.5103-5106.1999.
Abstract
Four proteins, PomA, PomB, MotX, and MotY, appear to be involved in force generation of the sodium-driven polar flagella of Vibrio alginolyticus. Among these, PomA and PomB seem to be associated and to form a sodium channel. By using antipeptide antibodies against PomA or PomB, we carried out immunoprecipitation to verify whether these proteins form a complex and examined the in vivo stabilities of PomA and PomB. As a result, we could demonstrate that PomA and PomB functionally interact with each other.
摘要
四种蛋白质，即PomA、PomB、MotX和MotY，似乎参与了溶藻弧菌钠驱动极鞭毛的力产生过程。其中，PomA和PomB似乎相互关联并形成一个钠通道。通过使用针对PomA或PomB的抗肽抗体，我们进行了免疫沉淀，以验证这些蛋白质是否形成复合物，并检测了PomA和PomB在体内的稳定性。结果，我们能够证明PomA和PomB在功能上相互作用。
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