Beer B E, Bailes E, Goeken R, Dapolito G, Coulibaly C, Norley S G, Kurth R, Gautier J P, Gautier-Hion A, Vallet D, Sharp P M, Hirsch V M
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA.
J Virol. 1999 Sep;73(9):7734-44. doi: 10.1128/JVI.73.9.7734-7744.1999.
Recently we reported the characterization of simian immunodeficiency virus (SIVlhoest) from a central African l'hoest monkey (Cercopithecus lhoesti lhoesti) that revealed a distant relationship to SIV isolated from a mandrill (SIVmnd). The present report describes a novel SIV (SIVsun) isolated from a healthy, wild-caught sun-tailed monkey (Cercopithecus lhoesti solatus), another member of the l'hoest superspecies. SIVsun replicated in a variety of human T-cell lines and in peripheral blood mononuclear cells of macaques (Macaca spp.) and patas monkeys (Erythrocebus patas). A full-length infectious clone of SIVsun was derived, and genetic analysis revealed that SIVsun was most closely related to SIVlhoest, with an amino acid identity of 71% in Gag, 73% in Pol, and 67% in Env. This degree of similarity is reminiscent of that observed between SIVagm isolates from vervet, grivet, and tantalus species of African green monkeys. The close relationship between SIVsun and SIVlhoest, despite their geographically distinct habitats, is consistent with evolution from a common ancestor, providing further evidence for the ancient nature of the primate lentivirus family. In addition, this observation leads us to suggest that the SIVmnd lineage should be designated the SIVlhoest lineage.
最近我们报道了从一只中非洛氏猴（Cercopithecus lhoesti lhoesti）中鉴定出的猿猴免疫缺陷病毒（SIVlhoest），该病毒与从山魈中分离出的SIV（SIVmnd）存在较远的亲缘关系。本报告描述了一种从健康的野生捕获太阳尾猴（Cercopithecus lhoesti solatus）中分离出的新型SIV（SIVsun），太阳尾猴是洛氏猴超种的另一个成员。SIVsun能在多种人类T细胞系以及猕猴（Macaca spp.）和赤猴（Erythrocebus patas）的外周血单个核细胞中复制。获得了SIVsun的全长感染性克隆，基因分析表明SIVsun与SIVlhoest关系最为密切，在Gag中的氨基酸同一性为71%，在Pol中为73%，在Env中为67%。这种相似程度让人联想到从非洲绿猴的黑长尾猴、灰长尾猴和白臀长尾猴物种中分离出的SIVagm之间的相似性。尽管SIVsun和SIVlhoest的栖息地在地理上不同，但它们之间的密切关系与共同祖先的进化一致，为灵长类慢病毒家族的古老性质提供了进一步证据。此外，这一观察结果使我们建议将SIVmnd谱系指定为SIVlhoest谱系。
