腺病毒介导的针对c-myb信使核糖核酸的核酶表达在体内抑制平滑肌细胞增殖和新生内膜形成。
Adenovirus-mediated expression of a ribozyme to c-myb mRNA inhibits smooth muscle cell proliferation and neointima formation in vivo.
作者信息
Macejak D G, Lin H, Webb S, Chase J, Jensen K, Jarvis T C, Leiden J M, Couture L
机构信息
Ribozyme Pharmaceuticals, Inc., Boulder, Colorado 80301, USA.
出版信息
J Virol. 1999 Sep;73(9):7745-51. doi: 10.1128/JVI.73.9.7745-7751.1999.
Abstract
Smooth muscle cell (SMC) proliferation is an important component of restenosis in response to injury after balloon angioplasty. Inhibition of proliferation in vivo can limit neointima hyperplasia in animal models of restenosis. Ribozymes against c-myb mRNA have been shown to be effective inhibitors of SMC proliferation in vitro. The effectiveness of adenovirus as a gene therapy vector in animal models of restenosis is well documented. In order to test the utility of ribozymes to inhibit SMC proliferation by a gene therapy approach, recombinant adenovirus expressing ribozymes against c-myb mRNA was generated and tested both in vitro and in vivo. This adenovirus ribozyme vector is shown to inhibit SMC proliferation in culture and neointima formation in a rat carotid artery balloon injury model of restenosis.
摘要
平滑肌细胞（SMC）增殖是球囊血管成形术后损伤引起再狭窄的一个重要组成部分。体内增殖抑制可限制再狭窄动物模型中的新生内膜增生。针对c-myb mRNA的核酶已被证明是体外SMC增殖的有效抑制剂。腺病毒作为基因治疗载体在再狭窄动物模型中的有效性已有充分记录。为了通过基因治疗方法测试核酶抑制SMC增殖的效用，构建了表达针对c-myb mRNA的核酶的重组腺病毒，并在体外和体内进行了测试。这种腺病毒核酶载体在培养中可抑制SMC增殖，并在大鼠颈动脉球囊损伤再狭窄模型中抑制新生内膜形成。
相似文献
1
Adenovirus-mediated expression of a ribozyme to c-myb mRNA inhibits smooth muscle cell proliferation and neointima formation in vivo.腺病毒介导的针对c-myb信使核糖核酸的核酶表达在体内抑制平滑肌细胞增殖和新生内膜形成。
J Virol. 1999 Sep;73(9):7745-51. doi: 10.1128/JVI.73.9.7745-7751.1999.
2
3
4
Optimizing the cell efficacy of synthetic ribozymes. Site selection and chemical modifications of ribozymes targeting the proto-oncogene c-myb.优化合成核酶的细胞效能。靶向原癌基因c-myb的核酶的位点选择与化学修饰
J Biol Chem. 1996 Nov 15;271(46):29107-12. doi: 10.1074/jbc.271.46.29107.
5
Adenovirus-encoded hammerhead ribozyme to PDGF A-chain mRNA inhibits neointima formation after arterial injury.腺病毒编码的针对血小板衍生生长因子A链信使核糖核酸的锤头状核酶可抑制动脉损伤后的新生内膜形成。
J Vasc Res. 2004 Jul-Aug;41(4):305-13. doi: 10.1159/000078928. Epub 2004 Jun 10.
6
Dedicator of cytokinesis 2, a novel regulator for smooth muscle phenotypic modulation and vascular remodeling.胞质分裂调控因子2，一种平滑肌表型调节和血管重塑的新型调节因子。
Circ Res. 2015 May 8;116(10):e71-80. doi: 10.1161/CIRCRESAHA.116.305863. Epub 2015 Mar 18.
7
Adenovirus-mediated transfer of ribozyme targeting platelet-derived growth factor A-chain mRNA inhibits growth of vascular smooth muscle cells from spontaneously hypertensive rats.
J Cardiovasc Pharmacol. 2002 Jun;39(6):858-65. doi: 10.1097/00005344-200206000-00011.
8
Conditional expression of a dominant-negative c-Myb in vascular smooth muscle cells inhibits arterial remodeling after injury.血管平滑肌细胞中显性负性c-Myb的条件性表达可抑制损伤后的动脉重塑。
Circ Res. 2003 Feb 21;92(3):314-21. doi: 10.1161/01.res.0000056758.73215.5a.
9
Adenovirus-mediated over-expression of the cyclin/cyclin-dependent kinase inhibitor, p21 inhibits vascular smooth muscle cell proliferation and neointima formation in the rat carotid artery model of balloon angioplasty.腺病毒介导的细胞周期蛋白/细胞周期蛋白依赖性激酶抑制剂p21的过表达可抑制大鼠球囊血管成形术颈动脉模型中的血管平滑肌细胞增殖和新生内膜形成。
J Clin Invest. 1995 Nov;96(5):2260-8. doi: 10.1172/JCI118281.
10
Akt controls vascular smooth muscle cell proliferation in vitro and in vivo by delaying G1/S exit.Akt通过延迟G1/S期转换来控制体外和体内血管平滑肌细胞的增殖。
Circ Res. 2003 Nov 28;93(11):1059-65. doi: 10.1161/01.RES.0000105086.31909.1B. Epub 2003 Nov 6.
引用本文的文献
1
The cell cycle: a critical therapeutic target to prevent vascular proliferative disease.细胞周期：预防血管增殖性疾病的关键治疗靶点。
Can J Cardiol. 2006 Feb;22 Suppl B(Suppl B):41B-55B. doi: 10.1016/s0828-282x(06)70986-2.
2
RelB-p50 NF-kappa B complexes are selectively induced by cytomegalovirus immediate-early protein 1: differential regulation of Bcl-x(L) promoter activity by NF-kappa B family members.巨细胞病毒立即早期蛋白1选择性诱导RelB-p50核因子-κB复合物：核因子-κB家族成员对Bcl-x(L)启动子活性的差异调控
J Virol. 2002 Jun;76(11):5737-47. doi: 10.1128/jvi.76.11.5737-5747.2002.
3
Catalytic DNAs as potential therapeutic agents and sequence-specific molecular tools to dissect biological function.催化性DNA作为潜在的治疗剂和用于剖析生物学功能的序列特异性分子工具。
J Clin Invest. 2000 Nov;106(10):1189-95. doi: 10.1172/JCI11620.
4
Ribozyme rescue of photoreceptor cells in P23H transgenic rats: long-term survival and late-stage therapy.核酶挽救P23H转基因大鼠光感受器细胞：长期存活与晚期治疗
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11488-93. doi: 10.1073/pnas.210319397.
本文引用的文献
1
Ribozyme rescue of photoreceptor cells in a transgenic rat model of autosomal dominant retinitis pigmentosa.在常染色体显性视网膜色素变性转基因大鼠模型中核酶对光感受器细胞的挽救作用
Nat Med. 1998 Aug;4(8):967-71. doi: 10.1038/nm0898-967.
2
Anti-gene therapy: the use of ribozymes to inhibit gene function.反基因疗法：利用核酶抑制基因功能。
Trends Genet. 1996 Dec;12(12):510-5. doi: 10.1016/s0168-9525(97)81398-4.
3
Histopathology of in-stent restenosis in patients with peripheral artery disease.外周动脉疾病患者支架内再狭窄的组织病理学
Circulation. 1997 Apr 15;95(8):1998-2002. doi: 10.1161/01.cir.95.8.1998.
4
5
Adenovirus-mediated expression of ribozymes in mice.腺病毒介导的核酶在小鼠中的表达。
J Virol. 1996 May;70(5):3153-8. doi: 10.1128/JVI.70.5.3153-3158.1996.
6
Nuclease-resistant ribozymes decrease stromelysin mRNA levels in rabbit synovium following exogenous delivery to the knee joint.耐核酸酶核酶在经膝关节外源性递送后可降低兔滑膜中基质溶解素mRNA水平。
Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):754-8. doi: 10.1073/pnas.93.2.754.
7
Methylphosphate cap structure increases the stability of 7SK, B2 and U6 small RNAs in Xenopus oocytes.磷酸甲酯帽结构增加了非洲爪蟾卵母细胞中7SK、B2和U6小RNA的稳定性。
Nucleic Acids Res. 1993 Oct 11;21(20):4756-61. doi: 10.1093/nar/21.20.4756.
8
Single intraluminal delivery of antisense cdc2 kinase and proliferating-cell nuclear antigen oligonucleotides results in chronic inhibition of neointimal hyperplasia.腔内单次递送反义cdc2激酶和增殖细胞核抗原寡核苷酸可导致对内膜增生的长期抑制。
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8474-8. doi: 10.1073/pnas.90.18.8474.
9
Gene therapy for vascular smooth muscle cell proliferation after arterial injury.动脉损伤后血管平滑肌细胞增殖的基因治疗。
Science. 1994 Aug 5;265(5173):781-4. doi: 10.1126/science.8047883.
10
In vivo suppression of injury-induced vascular smooth muscle cell accumulation using adenovirus-mediated transfer of the herpes simplex virus thymidine kinase gene.利用腺病毒介导的单纯疱疹病毒胸苷激酶基因转移对损伤诱导的血管平滑肌细胞积聚进行体内抑制。
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10732-6. doi: 10.1073/pnas.91.22.10732.
Zotero 插件