风疹病毒非结构蛋白P150的细胞内分布
Intracellular distribution of rubella virus nonstructural protein P150.
作者信息
Kujala P, Ahola T, Ehsani N, Auvinen P, Vihinen H, Kääriäinen L
机构信息
Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
出版信息
J Virol. 1999 Sep;73(9):7805-11. doi: 10.1128/JVI.73.9.7805-7811.1999.
Abstract
Antiserum prepared against an amino-terminal fragment of rubella virus (RUB) nonstructural polyprotein was used to study RUB-infected Vero cells. Replicase protein P150 was associated with vesicles and vacuoles of endolysosomal origin and later with large, convoluted, tubular membrane structures. Newly incorporated bromouridine was associated with the same structures and specifically with small membrane invaginations, spherules, indicating that these structures may be the sites of viral RNA synthesis.
摘要
用针对风疹病毒（RUB）非结构多蛋白氨基末端片段制备的抗血清来研究感染RUB的非洲绿猴肾细胞（Vero细胞）。复制酶蛋白P150与内溶酶体来源的囊泡和液泡相关，随后与大的、盘绕的管状膜结构相关。新掺入的溴尿苷与相同结构相关，特别是与小小膜小凹陷、小球相关，表明这些结构可能是病毒RNA合成的位点。
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