Kozlowski M, Schorey J, Portis T, Grigoriev V, Kornbluth J
Department of Pathology, St. Louis University School of Medicine, Veterans Administration Medical Center, MO 63106, USA.
J Immunol. 1999 Aug 15;163(4):1775-85.
NK cells are most effective in killing a broad spectrum of primary tumor cells after stimulation with cytokines. We have cloned a novel gene, designated NKLAM (for NK lytic-associated molecule), whose expression is associated with this cytokine-enhanced process. NKLAM expression is up-regulated in NK cells by IL-2 and IFN-beta. NKLAM is also selectively expressed by activated macrophages and CTL. Treatment of NK cells and CTL with NKLAM antisense oligonucleotides specifically decreases their cytolytic activity, while having no effect on cell growth. The NKLAM gene encodes a 62-kDa ring finger-containing protein that localizes to the cytoplasmic granules in NK cells. Further study of this gene may add to our understanding of cytotoxic processes common to NK cells, CTL, and activated macrophages.
自然杀伤细胞（NK细胞）在用细胞因子刺激后，在杀伤多种原发性肿瘤细胞方面最为有效。我们克隆了一个新基因，命名为NKLAM（自然杀伤细胞溶解相关分子），其表达与这种细胞因子增强的过程相关。白细胞介素-2（IL-2）和干扰素-β（IFN-β）可使NKLAM在NK细胞中的表达上调。活化的巨噬细胞和细胞毒性T淋巴细胞（CTL）也选择性表达NKLAM。用NKLAM反义寡核苷酸处理NK细胞和CTL可特异性降低它们的细胞溶解活性，而对细胞生长没有影响。NKLAM基因编码一种含62 kDa环指结构的蛋白质，定位于NK细胞的细胞质颗粒中。对该基因的进一步研究可能会增进我们对NK细胞、CTL和活化巨噬细胞共有的细胞毒性过程的理解。
