Salle B, Lornage J, Demirci B, Vaudoyer F, Poirel M T, Franck M, Rudigoz R C, Guerin J F
Hôpital de la Croix Rousse, Lyon, France.
Fertil Steril. 1999 Aug;72(2):366-70. doi: 10.1016/s0015-0282(99)00238-1.
To evaluate the effects of freezing, thawing, and autograft of a hemi-ovary on steroid secretion, endometrial maturation, and ovarian histology in ewes.
Experimental animal study.
Laboratoire de Zootechnie, Ecole Nationale Vétérinaire, Marcy l'Etoile, France.
ANIMAL(S): Six lambs aged 6 months to 1 year old.
INTERVENTION(S): Hemi-ovaries were prepared and frozen from the right ovary of six lambs and autografted 4 weeks later to the contralateral ovarian hilus. The autografts and the uterus were recovered 1 year later. Blood tests were performed each week to measure P concentration.
MAIN OUTCOME MEASURE(S): Number of primordial follicles; levels of plasma P.
RESULT(S): Histologic examination of ovarian slices after freezing showed no destruction of primordial, primary, secondary, or cavitary follicles. The frozen ovarian autograft showed good recovery of the macroscopic and microscopic ovarian structure. After autografting, histologic examination revealed primordial to cavitary follicles. Secretion of P started to rise 4 weeks after the autograft. Histologic analysis of the endometrium showed numerous glands, vessels, and mucous secretion.
CONCLUSION(S): Frozen ovarian autografts achieved P secretion and endometrial maturity.
评估半侧卵巢冷冻、解冻及自体移植对母羊类固醇分泌、子宫内膜成熟及卵巢组织学的影响。
实验动物研究。
法国马西莱图瓦勒国立兽医学院动物技术实验室。
6只6月龄至1岁的羔羊。
从6只羔羊的右侧卵巢制备半侧卵巢并冷冻，4周后自体移植至对侧卵巢门。1年后回收自体移植卵巢及子宫。每周进行血液检测以测定P浓度。
原始卵泡数量；血浆P水平。
冷冻后卵巢切片的组织学检查显示原始卵泡、初级卵泡、次级卵泡或腔卵泡均未被破坏。冷冻卵巢自体移植显示卵巢宏观和微观结构恢复良好。自体移植后，组织学检查发现有原始卵泡至腔卵泡。自体移植4周后P分泌开始增加。子宫内膜的组织学分析显示有大量腺体、血管和黏液分泌。
冷冻卵巢自体移植实现了P分泌及子宫内膜成熟。
