Salle B, Lornage J, Franck M, Isoard L, Rudigoz R C, Guerin J F
Centre d'Aide Médicale à la Procréation, Service de Gynécologie Obstétrique, Hôpital de la Croix Rousse, Lyon, France.
Fertil Steril. 1998 Jul;70(1):124-8. doi: 10.1016/s0015-0282(98)00095-8.
To evaluate the effects of freezing and thawing on the histologic changes in ovarian fragments from sheep and to determine the feasibility of ovarian autografts.
Histologic evaluation of follicles that survived after freezing at -196 degrees C for 2 weeks. Histologic evaluation of ovarian fragments 6 months after the autografts.
Laboratoire de Zootechnie, Ecole National Véterinaire, Marcy l'Etoile, France.
ANIMAL(S): Six ewes aged 6 months to 1 year.
INTERVENTION(S): Cortical fragments were prepared from the right ovary of 6 lambs and were grafted immediately to the contralateral ovarian hilus or were cooled slowly to -196 degrees C in medium containing dimethyl sulfoxide for 2 weeks. The autografts were recovered 6 months later.
RESULT(S): Histologic examination of ovarian slices after freezing showed no destruction of primordial, primary, secondary, or cavitary follicles. The ovarian autograft showed good recovery of the macroscopic and microscopic ovarian structure. After autografting, histologic examination revealed primordial to cavitary follicles.
CONCLUSION(S): Freezing of ovarian fragments is possible without damaging the follicles. Ovarian autografts showed good recovery of ovarian structure.
评估冻融对绵羊卵巢组织切片组织学变化的影响，并确定卵巢自体移植的可行性。
对在-196℃冷冻2周后存活的卵泡进行组织学评估。对自体移植6个月后的卵巢组织切片进行组织学评估。
法国马西耶图瓦勒国立兽医学院动物技术实验室。
6只年龄在6个月至1岁之间的母羊。
从6只羔羊的右侧卵巢制备皮质组织切片，立即移植到对侧卵巢门，或在含二甲亚砜的培养基中缓慢冷却至-196℃，持续2周。6个月后取出自体移植物。
冷冻后卵巢切片的组织学检查显示，原始卵泡、初级卵泡、次级卵泡或腔卵泡均未被破坏。卵巢自体移植物显示出宏观和微观卵巢结构的良好恢复。自体移植后，组织学检查发现有从原始卵泡到腔卵泡的存在。
卵巢组织切片冷冻是可行的，不会损害卵泡。卵巢自体移植物显示出卵巢结构的良好恢复。
