Ashman Robert B, Fulurija Alma, Papadimitriou John M
Pathology Department, University of Western Australia, Nedlands, WA 6009, Australia.
School of Dentistry, University of Queensland, Brisbane, Qld 4072, Australia.
Microbiology (Reading). 1999 Jul;145 ( Pt 7):1631-1640. doi: 10.1099/13500872-145-7-1631.
The role of T lymphocytes in host responses to sublethal systemic infection with Candida albicans was evaluated by mAb depletion of CD4+ and CD8+ cells from BALB/c and CBA/CaH mice, which develop mild and severe tissue damage, respectively. Depletion of CD4+ lymphocytes from BALB/c mice markedly increased tissue damage, but did not alter the course of infection. In CBA/CaH mice, depletion of CD4+ cells abrogated tissue destruction in both brain and kidney at day 4 after infection, and significantly decreased fungal colonization in the brain. However, the severity of tissue lesions increased relative to controls from day 8 onwards. A small increase in tissue damage was evident in both mouse strains after depletion of CD8+ cells. There were no major differences between days 4 and 8 after infection in cDNA cytokine profiles of CD4+ lymphocytes from either BALB/c or CBA/CaH mice. After passive transfer into infected syngeneic recipients, spleen cells from infected CBA/CaH mice markedly increased tissue damage when compared to controls, and also caused a significant increase in fungal colonization in the brain. A similar transfer in BALB/c mice increased the number of inflammatory cells in and around the lesions, but had no effect on the fungal burden in brain and kidney. The data demonstrate that both CD4+ and CD8+ lymphocytes contribute to the reduction of tissue damage after systemic infection with C. albicans, and that the development and expression of CD4+ lymphocyte effector function is influenced by the genetic background of the mouse.
通过用单克隆抗体清除BALB/c和CBA/CaH小鼠的CD4⁺和CD8⁺细胞，评估了T淋巴细胞在宿主对白念珠菌亚致死性全身感染反应中的作用，这两种小鼠分别会发生轻度和重度组织损伤。从BALB/c小鼠中清除CD4⁺淋巴细胞显著增加了组织损伤，但未改变感染进程。在CBA/CaH小鼠中，感染后第4天清除CD4⁺细胞可消除脑和肾中的组织破坏，并显著减少脑中的真菌定植。然而，从第8天起，相对于对照组，组织损伤的严重程度增加。清除CD8⁺细胞后，两种小鼠品系的组织损伤均有轻微增加。在感染后第4天和第8天，BALB/c或CBA/CaH小鼠的CD4⁺淋巴细胞的cDNA细胞因子谱没有主要差异。将感染的CBA/CaH小鼠的脾细胞被动转移到同基因感染受体中后，与对照组相比，显著增加了组织损伤，并且还导致脑中的真菌定植显著增加。在BALB/c小鼠中进行类似的转移增加了病变内和周围的炎症细胞数量，但对脑和肾中的真菌负荷没有影响。数据表明，CD4⁺和CD8⁺淋巴细胞均有助于减少白念珠菌全身感染后的组织损伤，并且CD4⁺淋巴细胞效应功能的发育和表达受小鼠遗传背景的影响。
