Abaitua Fernando, Rementería Aitor, Millan Rosario San, Eguzkiza Ainhoa, Rodriguez Jose Antonio, Pontón José, Sevilla María Jesús
Microbiology (Reading). 1999 Jul;145 ( Pt 7):1641-1647. doi: 10.1099/13500872-145-7-1641.
The in vitro effect of nitric oxide (NO) and nitrite on blastoconidia and hyphae of Candida albicans was studied. Sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) were used as NO donors. Both minimal and complex media at two pH values, 7.0 and 4.5, were used for the assays. Blastoconidia were more susceptible than hyphae to NO. The NO effect on blastoconidia was greater at acidic pH. Nitrite affected the viability of blastoconidia in complex medium. The percentage germination and the relative rate of elongation of hyphae were both enhanced when NO was present in acidic conditions.
研究了一氧化氮（NO）和亚硝酸盐对白色念珠菌芽生孢子和菌丝的体外作用。硝普钠（SNP）和S-亚硝基-N-乙酰青霉胺（SNAP）用作NO供体。在两种pH值（7.0和4.5）下的基础培养基和复合培养基都用于试验。芽生孢子比菌丝对NO更敏感。在酸性pH条件下，NO对芽生孢子的作用更大。亚硝酸盐影响复合培养基中芽生孢子的活力。当在酸性条件下存在NO时，芽生孢子的发芽率和菌丝的相对伸长率均提高。
