Maluszynska G M, Krachler B, Sundqvist T
Department of Medical Microbiology, Faculty of Health Sciences, University of Linköping, Sweden.
FEMS Microbiol Lett. 1998 Sep 15;166(2):249-55. doi: 10.1111/j.1574-6968.1998.tb13898.x.
Moraxella catarrhalis is sensitive to NO generators, e.g. S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SNP), but can spontaneously develop higher SNP tolerance. Using SDS-PAGE of outer membrane proteins and immunoblotting for serum albumin, we found that the wild strain bound more blood-medium-derived albumin than the SNP-resistant variant did. There was a negative correlation between NO tolerance and the presence of serum albumin in the medium. We suggest that M. catarrhalis can change its surface properties to avoid binding albumin and thereby increase its resistance to NO. Growth of Moraxella is affected by iron, and that may have influenced our results. Using chrome azurol S plates as an indicator, we noted that both albumin and SNP have a strong affinity for iron(III).
卡他莫拉菌对一氧化氮（NO）生成剂敏感，例如亚硝基 - N - 乙酰青霉胺（SNAP）和硝普钠（SNP），但它能自发产生对SNP更高的耐受性。通过外膜蛋白的十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）和血清白蛋白免疫印迹法，我们发现野生菌株比耐SNP变异株结合更多来自血液培养基的白蛋白。培养基中NO耐受性与血清白蛋白的存在呈负相关。我们认为卡他莫拉菌可以改变其表面性质以避免结合白蛋白，从而增加其对NO的抗性。莫拉菌的生长受铁的影响，这可能影响了我们的结果。以铬天青S平板作为指示剂，我们注意到白蛋白和SNP对铁（III）都有很强的亲和力。
