Mort J S, Buttle D J
Joint Diseases Laboratory, Shriners Hospital for Children, Montreal, Quebec, Canada.
Mol Pathol. 1999 Feb;52(1):11-8. doi: 10.1136/mp.52.1.11.
The hydrolysis of peptide bonds is an integral part of most physiological and pathological processes, yet knowledge is often lacking as to which peptide bonds are cleaved, in which protein substrates, in which order, and by which proteolytic enzymes. An increase in our understanding of these processes will enhance understanding of the pathogenesis underlying many diseases and might aid in the recognition of new targets for therapeutic intervention. This article reviews the development, design, and use of antibodies for the detection of specific peptide bond cleavage events, and describes how the application of such antibodies can increase our understanding of the roles played by proteolytic enzymes in physiology and pathology.
肽键的水解是大多数生理和病理过程中不可或缺的一部分，然而，对于哪些肽键被切割、在哪些蛋白质底物中、以何种顺序以及由哪些蛋白水解酶进行切割，我们往往缺乏了解。增进对这些过程的理解将有助于加深对许多疾病发病机制的认识，并可能有助于识别新的治疗干预靶点。本文综述了用于检测特定肽键切割事件的抗体的开发、设计和应用，并描述了此类抗体的应用如何能增进我们对蛋白水解酶在生理和病理过程中所起作用的理解。
