Gluschankof P, Cohen P
Université Pierre et Marie Curie, Alliée à l'Institut National de la Santé et de la Recherche Médicale, Paris, France.
Neurochem Res. 1987 Oct;12(10):951-8. doi: 10.1007/BF00966318.
Selective and limited proteolysis is a key step in the post-translational modification of peptide hormone precursors. This process appears to involve a proteolytic machinery including highly specific endoproteases. Some of the enzyme systems possibly involved in the processing of pro-neuropeptides will be described and their mechanism of action discussed. Special emphasis will be on the following: i) the physico-chemical characteristics of proteolytic enzymes which are believed to be involved in the processing of some of these polypeptide hormone precursors; ii) the bio-specificity of these enzymes toward the substrates; iii) the importance of both secondary and tertiary structures of the cleavage domain in recognition by the selective proteases. These properties will be discussed in connection with the possible importance of the maturation enzymes in the in vivo regulation of hormone biosynthesis.
选择性和有限的蛋白水解是肽激素前体翻译后修饰的关键步骤。这一过程似乎涉及一种蛋白水解机制，其中包括高度特异性的内切蛋白酶。将描述一些可能参与前神经肽加工的酶系统，并讨论它们的作用机制。特别强调以下几点：i）据信参与某些多肽激素前体加工的蛋白水解酶的物理化学特性；ii）这些酶对底物的生物特异性；iii）切割结构域的二级和三级结构在选择性蛋白酶识别中的重要性。将结合成熟酶在体内激素生物合成调节中的可能重要性来讨论这些特性。
