Lee N S, Nomura Y, Miyazaki T
Faculty of Bioresources, Mie University, Japan.
Dis Aquat Organ. 1999 Jun 23;37(1):13-21. doi: 10.3354/dao037013.
Since the late 1980s, a birnaviral gill disease has been occurring in Japanese eels Anguilla japonica reared in warmwater ponds in western regions in Japan. Diseased eels mostly displayed marked formations of aneurysmal hematomas within gill lamellae and high mortalities. Histological examination revealed necrosis of pillar cells and subsequent aggregation of erythrocytes inside the lamellar capillaries, and proliferation of interlamellar epithelia onto the lamellae. Gastric gland cells were also necrotized. Electron microscopy revealed birnavirus infection in lamellar pillar cells. The causative birnavirus was isolated and cultured in fish cell lines and was found to be related to an infectious pancreatic necrosis virus (IPNV) Sp serotype by neutralization tests. The viral pathogenicity was confirmed by the results of histopathological examinations and infectivity experiments.
自20世纪80年代末以来，日本西部地区温水池塘养殖的日本鳗鲡（Anguilla japonica）中一直出现一种双RNA病毒鳃病。患病鳗鱼大多在鳃小片内出现明显的动脉瘤性血肿形成，死亡率很高。组织学检查显示柱状细胞坏死，随后红细胞在片状毛细血管内聚集，片间上皮细胞在鳃小片上增殖。胃腺细胞也发生坏死。电子显微镜检查显示鳃小片柱状细胞中有双RNA病毒感染。分离出致病双RNA病毒并在鱼类细胞系中培养，通过中和试验发现其与传染性胰腺坏死病毒（IPNV）Sp血清型有关。组织病理学检查和感染性实验结果证实了病毒的致病性。
