Califano J V, Schifferle R E, Gunsolley J C, Best A M, Schenkein H A, Tew J G
Clinical Research Center for Periodontal Diseases, School of Dentistry, Virginia Commonwealth University, Richmond 23298-0566, USA.
J Periodontol. 1999 Jul;70(7):730-5. doi: 10.1902/jop.1999.70.7.730.
Six serotypes of Porphyromonas gingivalis have recently been described. We sought to test the hypothesis that serotype specific carbohydrates from these strains are important antigens that elicit potent immune responses.
Serum concentrations of IgG reactive with P. gingivalis serotypes K1-K6 were determined for 28 adult (AP) and 28 generalized early-onset (G-EOP) periodontitis patients previously determined to be seropositive for a broken cell preparation of P. gingivalis. To confirm relationships suggested for K1, K2, and K6 in the analysis of initial data, the study population was increased to 133.
Frequency of seropositivity for the 6 serotypes ranged from 26 to 54% of subjects. IgG concentrations ranged from 0 to 453 microg/ml with many subjects seropositive to more than one serotype. Concentrations for the subset of patients who was seropositive were high (mean responses ranged from 20 to 105 microg/ml for the 6 serotypes). Significant correlations between seropositivity to serotypes K1 and K5 as well as between K5 and K6 were found.
We examined the relationship of diagnosis, race, gender, smoking, probing depth, attachment loss, and antibody reaction with the P. gingivalis serotypes by analysis of variance. Initial findings suggested potential relationships between diagnosis, smoking, race, gender, and antibody reactive with serotypes K1, K2, and K6. A significant relationship did exist between smoking and decreased antibody reactive with P. gingivalis serotype K2. No other relationships were substantiated. We also examined the IgG subclass distribution and found that responses were almost exclusively IgG2. These data support the concept that antibody responses to all 6 serotypes are common in both AP and G-EOP and that these K serotype carbohydrates elicit potent IgG2 responses.
最近已描述了牙龈卟啉单胞菌的六种血清型。我们试图验证这样一种假设，即来自这些菌株的血清型特异性碳水化合物是引发强烈免疫反应的重要抗原。
测定了28名成年（AP）和28名广泛早发性（G-EOP）牙周炎患者血清中与牙龈卟啉单胞菌血清型K1-K6反应的IgG浓度，这些患者先前已被确定对牙龈卟啉单胞菌破碎细胞制剂血清学呈阳性。为了在初始数据分析中确认所提示的K1、K2和K6之间的关系，将研究人群增加到133人。
6种血清型的血清学阳性频率在受试者的26%至54%之间。IgG浓度范围为0至453微克/毫升，许多受试者对一种以上血清型血清学呈阳性。发现血清型K1和K5之间以及K5和K6之间血清学阳性存在显著相关性。
我们通过方差分析研究了诊断、种族、性别、吸烟、探诊深度、附着丧失以及抗体反应与牙龈卟啉单胞菌血清型之间的关系。初步研究结果提示诊断、吸烟、种族、性别与血清型K1、K2和K6抗体反应之间可能存在关系。吸烟与牙龈卟啉单胞菌血清型K2抗体反应降低之间确实存在显著关系。未证实其他关系。我们还研究了IgG亚类分布，发现反应几乎均为IgG2。这些数据支持这样一种概念，即AP和G-EOP患者对所有6种血清型的抗体反应均常见，且这些K血清型碳水化合物引发强烈的IgG2反应。
