Morris J L, Zhu B S, Gibbins I L, Blessing W W
Department of Anatomy and Histology, Flinders University of South Australia, Adelaide, South Australia 5001, Australia.
J Comp Neurol. 1999 Sep 13;412(1):147-60.
We have characterised sympathetic neurons projecting to a range of cutaneous and striated muscle vascular targets in the pinna of the rabbit ear by examining neurotransmitter-related enzymes and peptides in perivascular axons and in somata identified by retrograde axonal tracing. Fast Blue was injected into one of seven sites in each pinna (n = 21 pinnae). The soma cross-sectional area and immunoreactivity (IR) for tyrosine hydroxylase (TH) and neuropeptide Y (NPY) were determined for each of 2,041 retrogradely labelled neurons in the ipsilateral superior cervical ganglion (SCG) or stellate ganglion (StG). Larger neurons in the SCG with TH-IR but not NPY-IR projected predominantly to veins along the medial edge of the pinna. Larger neurons in the StG with TH-IR but not NPY-IR projected predominantly to arteries and veins in the tip and lateral edge of the pinna. Smaller neurons in the SCG with IR to both TH and NPY projected predominantly to arteries in the striated muscles at the base of the ear. The smallest retrogradely labelled neurons in the SCG or StG lacked TH-IR but contained NPY-IR and projected almost exclusively to arterial vessels in the lateral muscle at the base of the ear. Thus, somata of sympathetic neurons projecting to cutaneous versus striated muscle vessels or to different regions of the cutaneous bed could be distinguished by a combination of location, size, and immunohistochemical profile. Consequently, regulation of blood flow within the rabbit ear is likely to involve coordination between neuronal pathways containing neurochemically and morphologically distinct populations of sympathetic neurons.
我们通过检查血管周围轴突和经逆行轴突追踪鉴定的胞体中的神经递质相关酶和肽，对投射到兔耳廓一系列皮肤和横纹肌血管靶点的交感神经元进行了特征描述。将快蓝注射到每个耳廓的七个部位之一（n = 21个耳廓）。对同侧颈上神经节（SCG）或星状神经节（StG）中2041个逆行标记神经元中的每一个，测定其胞体横截面积以及酪氨酸羟化酶（TH）和神经肽Y（NPY）的免疫反应性（IR）。SCG中较大的具有TH免疫反应性但无NPY免疫反应性的神经元主要投射到耳廓内侧边缘的静脉。StG中较大的具有TH免疫反应性但无NPY免疫反应性的神经元主要投射到耳廓尖端和外侧边缘的动脉和静脉。SCG中对TH和NPY均有免疫反应性的较小神经元主要投射到耳基部横纹肌中的动脉。SCG或StG中最小的逆行标记神经元缺乏TH免疫反应性但含有NPY免疫反应性，几乎专门投射到耳基部外侧肌肉中的动脉血管。因此，投射到皮肤与横纹肌血管或皮肤床不同区域的交感神经元的胞体，可以通过位置、大小和免疫组织化学特征的组合来区分。因此，兔耳内的血流调节可能涉及含有神经化学和形态学上不同交感神经元群体的神经通路之间的协调。
