一种区分两个主要绵羊线粒体DNA单倍群的诊断检测方法。

A diagnostic assay discriminating between two major Ovis aries mitochondrial DNA haplogroups.

作者信息

Hiendleder S, Phua S H, Hecht W

机构信息

Department of Animal Breeding and Genetics, Justus-Liebig-University, Giessen, Germany.

出版信息

Anim Genet. 1999 Jun;30(3):211-3. doi: 10.1046/j.1365-2052.1999.00455.x.

DOI:10.1046/j.1365-2052.1999.00455.x

PMID:10442985

Abstract

Two major Ovis aries mitochondrial DNA (mtDNA) haplogroups have been described in independent studies. HinfI RFLP data of mitochondrial genomes from a large sample set (n = 239) indicated an ancient mutation which differentiates between the two mtDNA types. A completely determined sheep mtDNA sequence was used to assign this mutation to the COI gene and to develop a PCR based assay discriminating between the two phylogenetic branches. The haplogroup specificity of the mutation was further investigated in 26 randomly selected individuals. The animals were unequivocally assigned to their respective groups on the basis of the developed test and their complete control region sequences. The assay provides a rapid and economic means of discriminating between both major domestic sheep mtDNAs.

摘要

在独立研究中已描述了两个主要的绵羊线粒体DNA（mtDNA）单倍群。来自大量样本集（n = 239）的线粒体基因组的HinfI限制性片段长度多态性（RFLP）数据表明存在一个古老的突变，该突变可区分两种mtDNA类型。利用一个完全确定的绵羊mtDNA序列将此突变定位到细胞色素氧化酶亚基I（COI）基因，并开发了一种基于聚合酶链反应（PCR）的检测方法来区分两个系统发育分支。在26个随机选择的个体中进一步研究了该突变的单倍群特异性。根据所开发的检测方法及其完整的控制区序列，可明确地将这些动物归为各自的组。该检测方法提供了一种快速且经济的手段来区分两种主要的家养绵羊mtDNA。