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Use of I-Sce I to induce DNA double-strand breaks in Nicotiana.

作者信息

Puchta H

机构信息

Institut fur Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.

出版信息

Methods Mol Biol. 1999;113:447-51. doi: 10.1385/1-59259-675-4:447.

DOI:10.1385/1-59259-675-4:447
PMID:10443441
Abstract
摘要

相似文献

1
Use of I-Sce I to induce DNA double-strand breaks in Nicotiana.利用I-Sce I在烟草中诱导DNA双链断裂。
Methods Mol Biol. 1999;113:447-51. doi: 10.1385/1-59259-675-4:447.
2
Expression of I-Sce I in Drosophila to induce DNA double-strand breaks.
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Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination.植物通过同源重组修复基因组双链断裂时使用了两种不同但相关的机制。
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Chromosomal double-strand breaks introduced in mammalian cells by expression of I-Sce I endonuclease.
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Repair of DNA double strand breaks: in vivo biochemistry.DNA双链断裂的修复：体内生物化学
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7
The yeast I-Sce I meganuclease induces site-directed chromosomal recombination in mammalian cells.
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Analysis of recombinational repair of DNA double-strand breaks in mammalian cells with I-SceI nuclease.利用I-SceI核酸酶分析哺乳动物细胞中DNA双链断裂的重组修复
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Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease.通过位点特异性核酸内切酶在体内诱导DNA双链断裂，可增强植物细胞中的同源重组。
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Two unlinked double-strand breaks can induce reciprocal exchanges in plant genomes via homologous recombination and nonhomologous end joining.两个不连锁的双链断裂可通过同源重组和非同源末端连接在植物基因组中诱导相互交换。
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Efficient repair of genomic double-strand breaks by homologous recombination between directly repeated sequences in the plant genome.通过植物基因组中直接重复序列之间的同源重组实现基因组双链断裂的高效修复。
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