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The yeast I-Sce I meganuclease induces site-directed chromosomal recombination in mammalian cells.

作者信息

Choulika A, Perrin A, Dujon B, Nicolas J F

机构信息

Unité de Biologie Moléculaire du Développement, Institut Pasteur, Paris, France.

出版信息

C R Acad Sci III. 1994 Nov;317(11):1013-9.

PMID:7882137
Abstract

Double-strand breaks in genomic DNA stimulate recombination. Until now it was not possible to induce in vivo site-directed double-strand breaks in a mammalian chromosomal target. In this article we describe the use of I-Sce I meganuclease, a very rare cutter yeast endonuclease, to induce site-directed double-strand breaks mediated recombination. The results demonstrate the potential of the I-Sce I system for chromosome manipulation in mammalian cells.

摘要

相似文献

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The yeast I-Sce I meganuclease induces site-directed chromosomal recombination in mammalian cells.
C R Acad Sci III. 1994 Nov;317(11):1013-9.
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