Meske V, Albert F, Wehser R, Ohm T G
Institut für Anatomie, Universitätsklinikum Charité, Humboldt-Universität, Berlin, Federal Republic of Germany.
J Neural Transm (Vienna). 1999;106(5-6):537-48. doi: 10.1007/s007020050177.
The present study was undertaken in order to analyse the possibility of culturing post mortem derived human fibroblasts. The combination of post mortem fibroblasts with the autopsy proven and histopathologically staged brain will allow the correlative investigation of dynamic biochemical processes which are systemically underlying or accompanying a neurological and/or psychiatric disorder. These studies are limited in autopsy brain or are uncertain when the neuropathological status is lacking, i.e. when fibroblasts were obtained from living patients. Our examinations of human autopsy fibroblast and those under experimentally controlled post mortem conditions with rats clearly demonstrate that autopsy-derived fibroblasts can be reliably cultured. The cells grown displayed typical morphological and staining characteristics as well as pharmacological responsiveness. Even cells obtained from a 99 years old individual or an individual with a post mortem delay of 48 hours grew in our culture system.
本研究旨在分析培养死后获得的人成纤维细胞的可能性。将死后成纤维细胞与经尸检证实且组织病理学分期的大脑相结合，将有助于对动态生化过程进行相关研究，这些过程是神经系统和/或精神疾病的系统基础或伴随症状。这些研究在尸检大脑中受到限制，或者在缺乏神经病理学状态时（即从活体患者获取成纤维细胞时）存在不确定性。我们对人类尸检成纤维细胞以及在实验控制的大鼠死后条件下的成纤维细胞进行的检查清楚地表明，死后获得的成纤维细胞可以可靠地培养。培养出的细胞呈现出典型的形态和染色特征以及药理反应性。即使是从一名99岁个体或死后延迟48小时的个体获得的细胞，也能在我们的培养系统中生长。
