Banner Sun Health Research Institute, Sun City, AZ 85351, USA.
School of Biological and Health Systems Engineering, Arizona State University, 501 E. Tyler Mall, ECG 334A, Tempe, AZ 85287, USA.
Cells. 2020 Oct 30;9(11):2383. doi: 10.3390/cells9112383.
The Arizona Study of Aging and Neurodegenerative Disorders/Brain and Body Donation Program at Banner Sun Health Research Institute (BSHRI) is a longitudinal clinicopathological study with a current enrollment of more than 900 living subjects for aging and neurodegenerative disease research. Annual clinical assessments are done by cognitive and movement neurologists and neuropsychologists. Brain and body tissues are collected at a median postmortem interval of 3.0 h for neuropathological diagnosis and banking. Since 2018, the program has undertaken banking of scalp fibroblasts derived from neuropathologically characterized donors with Alzheimer's disease, Parkinson's disease, and other neurodegenerative diseases. Here, we describe the procedure development and cell characteristics from 14 male and 15 female donors (mean ± SD of age: 83.6 ± 12.2). Fibroblasts from explant cultures were banked at passage 3. The results of mRNA analysis showed positive expression of fibroblast activation protein, vimentin, fibronectin, and THY1 cell surface antigen. We also demonstrated that the banked fibroblasts from a postmortem elderly donor were successfully reprogramed to human-induced pluripotent stem cells (hiPSCs). Taken together, we have demonstrated the successful establishment of a human autopsy-derived fibroblast banking program. The cryogenically preserved cells are available for request at the program website of the BSHRI.
亚利桑那州衰老与神经退行性疾病研究/班纳太阳健康研究所脑与身体捐赠计划（BSHRI）是一项针对衰老和神经退行性疾病的纵向临床病理研究，目前有超过 900 名存活受试者参与该研究。每年由认知和运动神经病学家以及神经心理学家进行临床评估。在死后中位数 3.0 小时内收集脑组织和身体组织，以进行神经病理学诊断和存储。自 2018 年以来，该计划已开始存储从经神经病理学特征确定的患有阿尔茨海默病、帕金森病和其他神经退行性疾病的供体中提取的头皮成纤维细胞。在此，我们描述了 14 名男性和 15 名女性供体（年龄的平均值 ± 标准差：83.6 ± 12.2）的程序开发和细胞特征。从原代培养物中提取的成纤维细胞在第 3 代时进行存储。mRNA 分析结果显示成纤维细胞活化蛋白、波形蛋白、纤连蛋白和 THY1 细胞表面抗原呈阳性表达。我们还证明了来自老年尸检供体的存储成纤维细胞可以成功地被重编程为人诱导多能干细胞（hiPSC）。综上所述，我们已经成功建立了一个人类尸检来源的成纤维细胞存储计划。冷冻保存的细胞可在 BSHRI 的计划网站上申请。
