Ehrén I, Hosseini A, Herulf M, Lundberg J O, Wiklund N P
Department of Urology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
Urology. 1999 Aug;54(2):264-7. doi: 10.1016/s0090-4295(99)00120-x.
Nitric oxide (NO) measured in the gaseous phase has been shown to be a marker of inflammation in the urinary bladder. The NO content of air incubated in the bladder can be measured in an NO analyzer. The aim of our study was threefold: to evaluate whether NO can be measured in air incubated in a catheter balloon, to determine the optimal time of incubation, and to find the most suitable type of catheter.
The NO concentration in air introduced directly into the bladder and into the catheter balloon was measured in patients with and without bladder infections. The air was incubated for 5 to 60 minutes. NO concentration in the bladder of patients with interstitial cystitis was also analyzed. The diffusion rate of NO through silicon and latex catheters was studied.
Elevated NO levels were detected in the urinary bladder in patients with bladder inflammation due to infection or interstitial cystitis. A marked increase in NO concentration was found after just 5 minutes of incubation and continued to rise for up to 20 minutes, both in air taken directly from the bladder and from the catheter balloon. The NO diffusion rate into the balloons of silicon catheters was high; the recovery rate in latex catheters was poor.
Measurement of NO concentration in a silicon balloon catheter inserted into the urinary bladder is a fast, convenient, and reliable method to detect inflammation.
气相中测量的一氧化氮（NO）已被证明是膀胱炎症的标志物。膀胱中孵育的空气的NO含量可在NO分析仪中进行测量。我们研究的目的有三个：评估是否可以在导管球囊中孵育的空气中测量NO，确定最佳孵育时间，并找到最合适的导管类型。
在有和没有膀胱感染的患者中，测量直接引入膀胱和导管球囊的空气中的NO浓度。空气孵育5至60分钟。还分析了间质性膀胱炎患者膀胱中的NO浓度。研究了NO通过硅导管和乳胶导管的扩散速率。
在因感染或间质性膀胱炎引起膀胱炎症的患者的膀胱中检测到NO水平升高。在直接从膀胱和导管球囊中采集的空气中，孵育仅5分钟后NO浓度就显著增加，并持续上升至20分钟。NO扩散到硅导管球囊中的速率很高；乳胶导管中的回收率很差。
测量插入膀胱的硅球囊导管中的NO浓度是一种检测炎症的快速、方便且可靠的方法。
