Novella M L, Maldonado C, Aoki A, Coronel C E
Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Arch Androl. 1999 Jul-Aug;43(1):1-12. doi: 10.1080/014850199262689.
Effects of androgen status on the synthesis and secretion of rat caltrin have been studied by three different procedures: a) immunocytochemistry in seminal vesicle tissues; b) polyacrylamide gel electrophoresis and Western immunostaining of seminal vesicle secretion; and c) evaluation of trypsin inhibitory activity of the seminal vesicle secretion. Rat caltrin has been immunolocalized in cells of the secretory epithelium, specifically in the electron-lucent halo of secretory granules which store and transport proteins to the lumen. No caltrin immunoreaction was detected 14 days postcastration, and the ultrastructure of the epithelial cells was markedly altered. SDS-PAGE and Western blotting of the seminal vesicle secretion revealed alterations in the protein pattern and loss of the caltrin-related immunoreactive bands. The 54-kDa caltrin-precursor protein and the 6.2-kDa active caltrin were absent. Trypsin inhibitory activity of the seminal secretion was reduced about 50% in castrated animals. Daily testosterone administration restored both the protein pattern and immunoreactivity of the seminal vesicle secretion, and, as expected, reversed the morphological alterations of the gland after 7 days of treatment. Trypsin--inhibitor effect of the secretion also returned to normal levels after fourteen days of testosterone administration. Data suggest that the synthesis and secretion of caltrin are testosterone-dependent processes.
a）精囊组织的免疫细胞化学；b）精囊分泌物的聚丙烯酰胺凝胶电泳和 Western 免疫染色；c）精囊分泌物胰蛋白酶抑制活性的评估。大鼠钙调素已在分泌上皮细胞中免疫定位，具体位于储存和运输蛋白质至管腔的分泌颗粒的电子透明晕中。去势后 14 天未检测到钙调素免疫反应，上皮细胞的超微结构明显改变。精囊分泌物的 SDS-PAGE 和 Western 印迹显示蛋白质模式改变以及与钙调素相关的免疫反应条带消失。54 kDa 的钙调素前体蛋白和 6.2 kDa 的活性钙调素缺失。去势动物的精液胰蛋白酶抑制活性降低约 50%。每日给予睾酮可恢复精囊分泌物的蛋白质模式和免疫反应性，并且如预期的那样，在治疗 7 天后逆转了腺体的形态改变。给予睾酮 14 天后，分泌物的胰蛋白酶抑制作用也恢复到正常水平。数据表明钙调素的合成与分泌是睾酮依赖性过程。
