Aumüller G, Seitz J
Histochem J. 1986 Jan;18(1):15-23. doi: 10.1007/BF01676193.
Immunoelectron microscopy of the rat seminal vesicle was performed using specific antibodies to secretory proteins. Proteins were precipitated from rat seminal vesicle secretion and were separated by SDS-polyacrylamide gel electrophoresis. Among the great number of bands the two most prominent bands were selected and designated SVS II and IV. Their apparent molecular weights were 48 kDa and 16.5 kDa respectively. The bands were excised from the gels and used for antibody production in rabbits. The respective antisera were used for immunohistochemical studies both at the light and electron microscopic levels in the rat seminal vesicle and the different prostatic lobes in infantile, adult and castrated animals. A positive immunoreaction was observed in seminal vesicle and lateral prostatic epithelium of the intact adult rat, while it was lacking in prepubertal and castrated animals. The subcellular distribution of both proteins was clearly different: SVS II was exclusively confined to the electron dense core of the secretory vacuoles, while SVS IV was detected only in the clear halo surrounding the central granule. It is suggested that the spatial arrangement of both proteins in the seminal vesicle secretion vacuole reflects a particular functional significance of each of these proteins. These proteins may serve as a tool in the study of regulation of androgen-dependent protein synthesis.
利用针对分泌蛋白的特异性抗体对大鼠精囊进行免疫电子显微镜检查。从大鼠精囊分泌物中沉淀蛋白质，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离。在众多条带中，选择了两条最明显的条带，分别命名为SVS II和IV。它们的表观分子量分别为48 kDa和16.5 kDa。从凝胶中切下这些条带，用于在兔体内制备抗体。各自的抗血清用于在光镜和电镜水平对大鼠精囊以及幼年、成年和去势动物的不同前列腺叶进行免疫组织化学研究。在完整成年大鼠的精囊和前列腺外侧上皮中观察到阳性免疫反应，而在青春期前和去势动物中则没有。两种蛋白质的亚细胞分布明显不同：SVS II仅局限于分泌泡的电子致密核心，而SVS IV仅在围绕中央颗粒的透明晕中检测到。有人认为，这两种蛋白质在精囊分泌泡中的空间排列反映了每种蛋白质的特定功能意义。这些蛋白质可作为研究雄激素依赖性蛋白质合成调控的工具。
