Sotogaku N, Endo K, Hirunuma R, Enomoto S, Ambe S, Ambe F
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
J Trace Elem Med Biol. 1999 Jul;13(1-2):1-6. doi: 10.1016/S0946-672X(99)80016-9.
The binding affinity of various trace elements to blood components and the pH-dependence of the binding affinity of the elements to serum proteins were examined using the radioactive multitracer technique. The binding affinity of 13 elements (Be, V, Mn, Zn, Se, Rb, Sr, Ce, Eu, Gd, Tm, Yb, and Lu) was simultaneously determined by gamma-ray spectrometry. The blood drawn from rats was separated into plasma, corpuscles, and erythrocyte ghosts. It was found that Be, Sr, Mn, and Zn bind highly to the plasma proteins. V and Se were highly bound to the corpuscles, and Se to the erythrocyte ghosts as well. Similar binding percentages of rare earth elements (Ce, Eu, Gd, Tm, Yb, and Lu) were found for each of the blood components, with the highest percentages being observed for plasma proteins. Albumin, beta-globulin, gamma-globulin, apotransferrin, and holotransferrin were examined in the study on the affinity of individual serum proteins. The pH dependence of the affinity of metal ions to the serum proteins in the pH range of 6.4-8.5 was examined using ultrafiltration and gamma-ray spectrometry. Each element showed a characteristic binding affinity to each serum protein, depending on pH. The results are discussed in terms of the chelating ability of metal ions and the nature of the serum proteins.
采用放射性多示踪技术研究了各种微量元素与血液成分的结合亲和力以及这些元素与血清蛋白结合亲和力的pH依赖性。通过γ射线能谱法同时测定了13种元素（铍、钒、锰、锌、硒、铷、锶、铈、铕、钆、铥、镱和镥）的结合亲和力。从大鼠采集的血液被分离成血浆、血细胞和红细胞影。结果发现，铍、锶、锰和锌与血浆蛋白高度结合。钒和硒与血细胞高度结合，硒也与红细胞影高度结合。对于每种血液成分，稀土元素（铈、铕、钆、铥、镱和镥）的结合百分比相似，其中血浆蛋白中的结合百分比最高。在关于单个血清蛋白亲和力的研究中，检测了白蛋白、β球蛋白、γ球蛋白、脱铁转铁蛋白和全转铁蛋白。使用超滤和γ射线能谱法研究了金属离子与血清蛋白在pH 6.4 - 8.5范围内亲和力的pH依赖性。每种元素对每种血清蛋白都表现出取决于pH的特征性结合亲和力。根据金属离子的螯合能力和血清蛋白的性质对结果进行了讨论。
